نتایج جستجو برای: baculovirus expression system

تعداد نتایج: 2988560  

2013
Su Xiao Jim F. White Michael J. Betenbaugh Reinhard Grisshammer Joseph Shiloach

Nowadays, baculovirus-infected insect cells and tetracycline-inducible mammalian cell lines (T-REx-293) are intensively used for G protein-coupled receptor (GPCR) production for crystallography purposes. Here we constructed a suspension T-REx-293 cell line to stably express an engineered neurotensin receptor 1 (NTS1) mutant and we quantitatively compared this cell line with the transient baculo...

2013
Feng Zhu Zhongyuan Shen Shengyan Xiao Yajie Yue Xuliang Fu Xudong Tang Li Xu Xijie Guo

The microsporidian spore wall proteins, as the main components of the spore wall, play a key role in spore adherence to host cells and in recognition of the parasite by the host during the invasion process. In this study, we used the Bac-to-Bac baculovirus expression system to express the spore wall protein SWP26, fused to enhanced green fluorescent protein (EGFP), in the silkworm BmN cell line...

Journal: :BioTechniques 2003
Y H Je B R Jin H W Park J Y Roh J H Chang S J Seo J A Olszewski D R O'Reilly S K Kang

Current baculovirus expression systems typically produce soluble proteins that accumulate within the infected insect cell or are secreted into the growth medium. A system has now been developed for the incorporation of foreign proteins, along with the matrix protein, polyhedrin, into baculovirus occlusion bodies. Initial studies showed that a recombinant virus expressing a translational fusion ...

Journal: :Journal of virology 1994
S E Crawford M Labbé J Cohen M H Burroughs Y J Zhou M K Estes

Rotaviruses are triple-layered particles that contain four major capsid proteins, VP2, VP4, VP6, and VP7, and two minor proteins, VP1 and VP3. We have cloned each of the rotavirus genes coding for a major capsid protein into the baculovirus expression system and expressed each protein in insect cells. Coexpression of different combinations of the rotavirus major structural proteins resulted in ...

2017
Natalia Inés Almasia María Paula Molinari Guillermo Andrés Maroniche Vanesa Nahirñak María Pilar Barrios Barón Oscar Alberto Taboga Cecilia Vazquez Rovere

BACKGROUND Snakin-1 (StSN1) is a broad-spectrum antimicrobial cysteine-rich peptide isolated from Solanum tuberosum. Its biotechnological potential has been already recognized since it exhibits in vivo antifungal and antibacterial activity. Most attempts to produce StSN1, or homologous peptides, in a soluble native state using bacterial, yeast or synthetic expression systems have presented prod...

Journal: :Protein expression and purification 2004
T Pechan P W K Ma D S Luthe

Several heterologous expression systems were tested for their ability to express a unique maize cysteine proteinase Mir1. A baculovirus-based expression system using Trichoplusia ni larvae as host resulted in the expression of Mir1 that was correctly processed and exhibited proteinase activity. Expression in Escherichia coli resulted in accumulation of Mir1, but it had limited solubility and en...

Journal: :Drug metabolism and disposition: the biological fate of chemicals 2002
Kenda A Marcucci Robin E Pearce Charles Crespi Dorothy T Steimel J Steven Leeder Andrea Gaedigk

Over 50 allelic variants of cytochrome P450 2D6 (CYP2D6) encoding fully functional, reduced-activity, or nonfunctional proteins have been described. Compared with Caucasians, studies in black populations demonstrate a tendency toward slower CYP2D6 activity, attributed in part to the presence of a variant allele associated with reduced activity, the CYP2D6*17 allele. To investigate the kinetic c...

Journal: :Biochemical and biophysical research communications 1995
C H Klaassen H G Swarts J J De Pont

The baculovirus expression system is suitable for functional expression of gastric H+,K(+)-ATPase. Expression of functional H+,K(+)-ATPase in Sf9 cells is accompanied by synthesis of large amounts of non-functional subunits. When H+,K(+)-ATPase is synthesised in the presence of 150-250 mM ethanol in the culture medium, two to threefold higher levels of functional H+,K(+)-ATPase are produced due...

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