OBJECTIVE The aim of this study was to detect the prevalence of selected bacterial species in intraoral sites of patients with chronic periodontitis (CP) using multiplex polymerase chain reaction (PCR). METHODOLOGY Samples were collected from the tongue dorsum, buccal mucosa, supragingival and subgingival plaque and saliva of 30 patients with untreated CP. Multiplex PCR was used to determine ...

Oral disease an on-going concern During the last century, there has been a world-wide surge of interest in veterinary dentistry. Interstitial gingivitis or so-called pyorrhoea alveolaris” was reported in dogs as far back as 1899. In 1939 the prevalence of dental disease and requirement for surgical intervention in dogs was also reported. Dental disease is certainly not a new “modern” problem in...

Background Dental plaque is one of the predominant causes of major oral diseases. Although mechanical and chemical methods are extensively followed to control the development of plaque, plaque-related diseases still persist. Therefore, this necessitates for alternative measures of plaque control, one such alternative is photodynamic antimicrobial chemotherapy (PACT). Materials and Methods Spl...

abstract biofilm is a complex community of microorganisms residing within a polysaccharide and/or protein matrix. biofilm can be produced by microorganisms such as bacteria and fungi. both gram positive and gram negative bacteria have this ability. resistance of biofilm to antimicrobial agents is becoming a global issue. bacterial biofilms are important in various aspects such as chronic human ...

A medium for detecting colonies of Actinomyces viscosus and Actinomyces naeslundii in dental plaque samples was developed. The medium (CNAC-20) contains 20.0 mug of 3CdSO4-8H2O per ml of Columbia CNA agar base. Laboratory strains of A. viscosus grew on CNAC-20 in characteristic round, white, smooth, opaque colonies. Increasing the cadmium concentration impaired the growth of some A. viscosus st...

BACKGROUND In ventral hernia surgery, mesh implants are used to reduce recurrence. Infection after mesh implantation can be a problem and rates around 6-10% have been reported. Bacterial colonization of mesh implants in patients without clinical signs of infection has not been thoroughly investigated. Molecular techniques have proven effective in demonstrating bacterial diversity in various env...

BACKGROUND There is an association between periodontal diseases and preterm delivery. AIM To assess the relationship between periodontal diseases, ascending bacterial infection and placental pathology with preterm delivery. PATIENTS AND METHODS A periodontal examination and collection of amniotic fluid and subgingival plaque samples were performed in women with preterm labor with intact mem...

Plaque-forming, galactose-transducing lambda strains have been isolated from lysogens in which bacterial genes have been removed from between the galactose operon and the prophage by deletion mutation.-A second class has been isolated starting with a lysogenic strain which carries a deletion of the genes to the right of the galactose operon and part of the prophage. This strain was lysogenized ...

Invasion and plaque formation in epithelial monolayers are routinely used to assess the virulence of Shigella flexneri, a causative agent of dysentery. A modified plaque assay was developed to identify factors contributing to the virulence of S. flexneri under the anaerobic conditions present in the colon. This assay demonstrated the importance of the ferrous iron transport system Feo, as well ...

α-Amylase-binding streptococci (ABS) are a heterogeneous group of commensal oral bacterial species that comprise a significant proportion of dental plaque microfloras. Salivary α-amylase, one of the most abundant proteins in human saliva, binds to the surface of these bacteria via specific surface-exposed α-amylase-binding proteins. The functional significance of α-amylase-binding proteins in o...