There is still an ongoing demand for a simple broad-spectrum molecular diagnostic assay for pathogenic bacteria. For this purpose, we developed a single-plex High Resolution Melt (HRM) assay that generates complex melt curves for bacterial identification. Using internal transcribed spacer (ITS) region as the phylogenetic marker for HRM, we observed complex melt curve signatures as compared to 1...

background: there is evidence of antibacterial properties of human chorioamniotic layer. however, the distinctive contribution of its individual parts, amniotic and chorionic membranes, to these effects is still unknown. the aim of present study was comparison of the antibacterial effects between amniotic and chorionic membranes. methods: chorioamniotic layer was removed from placenta belonging...