To carry out the immunological experiments on the serum of Multiple Sclerosis (MS) patients, based on a correlation between Borrelia burgdorferi infection and contracting MS autoimmune disease the outer surface protein D (OspD) of the bacterium was expressed and purified. A clone containing the OspD gene in pET11a expression vector under the control of T7 promoter was transformed to the bacteri...

Quorum sensing (QS), or cell-to-cell communication in bacteria, is achieved through the production and subsequent response to the accumulation of extracellular signal molecules called autoinductors. The main role of QS is regulation of production of virulence factors in bacteria. Bacterial pathogenicity is often manifested by the expression of various cell-associated and secreted virulence fact...

From how a single cell develops into a multicellular organism to how bacteria decide to go about their diet, single cells interpret the information encoded in their DNA and in their surrounding media in order to make life-changing decisions. Cellular decision making is ubiquitous in biology. As an example, most animals have the same set of genes encoded in their DNA. However, what sets them apa...

introduction bacterial meningitis is a known infectious disease which occurs at early ages and should be promptly diagnosed and treated. bacterial and aseptic meningitis are hard to be distinguished. therefore, physicians should be highly informed and experienced in this area. the main aim of this study was to suggest a system for distinguishing between bacterial and aseptic meningitis, using f...

Anthropogenic N deposition alters patterns of C and N cycling in temperate forests, where forest floor litter decomposition is a key process mediated by a diverse community of bacteria and fungi. To track forest floor decomposer activity we generated metatranscriptomes that simultaneously surveyed the actively expressed bacterial and eukaryote genes in the forest floor, to compare the impact of...