Showing a high sequence similarity, the evolutionary closely related bacterial poly(A) polymerases (PAP) and CCA-adding enzymes catalyze quite different reactions--PAP adds poly(A) tails to RNA 3'-ends, while CCA-adding enzymes synthesize the sequence CCA at the 3'-terminus of tRNAs. Here, two highly conserved structural elements of the corresponding Escherichia coli enzymes were characterized....

Lanthanides are relative newcomers to the field of cell biology metals; their specific incorporation into enzymes was only demonstrated in 2011, with isolation a bacterial lanthanide- and pyrroloquinoline quinone-dependent methanol dehydrogenase. Since that discovery, efforts many investigators have revealed lanthanide utilization is widespread environmentally important bacteria, parallel focus...

Bacterial monooxygenase enzymes catalyze a regiospecific single-step hydroxylation of diphenylacetylene to yield meta- and para-hydroxydiphenylacetylene.

An analysis of bacterial communities in soil samples from around the world reveals unexplored diversity in biosynthetic enzymes.

In modern medical processes, new antimicrobial drugs are being widely used in order to rapid and essential eradication of resistant bacterial strains. Broad-spectrum beta-lactamases are enzymes originated from mutations in the common plasmid genes mediators for beta-lactamases such as TEM-1, TEM-2, and SHV-1 are transferred among bacterial species. The genes coding the production of ESBL are of...

There are many molybdenum-containing enzymes distributed throughout the biosphere. The availability of molybdenum to biological systems is due to the high water solubility of oxidized forms of the metal. Molybdenum enzymes can be grouped on the basis of the structure of the metal centre. Three principal families of enzyme exist, with active sites consisting of (ppt)MoOS(OH) (the molybdenum hydr...

The use of physical maps for the description and comparison of bacterial genomes has dramatically increased in the last few years with the advent of powerful tools for the construction of physical maps of large DNA molecules. The availability of restriction enzymes that cut whole bacterial genomes only a few times together with the use of pulsed-field gel electrophoresis to resolve and size the...

Ratooning in sugarcane often leads to soil problems such as degradation, acidification, and soil-borne diseases that negatively impact agriculture output sustainability. Understanding the alteration bacterial communities, activities, their diversity connected plant under consecutive ratooning still needs be clarified. To address this gap, multidisciplinary approaches Illumina sequencing measure...

Environmental bacteria express a wide diversity of glycoside hydrolases (GH). Screening and characterization GH from metagenomic sources provides an insight into biomass degradation strategies non-cultivated prokaryotes. In the present report, we screened compost metagenome for lignocellulolytic activities identified six genes encoding enzymes belonging to family GH9 (GH9a-f). Three these (GH9b...

Glycine max, Mannosidase, Peribacteroid Space. Rhizobium Three of the major soluble partitioned pools in the symbiotic cell, namely the host cell cytoplasm, the cytoplasm of the bacterial symbiont and the space between the two partners (the peribacteroid space) were assayed for selected enzymes. W hilst alanine dehydrogenase was found almost totally in the bacterial cell cytoplasm, other enzyme...