A lipase producing bacterium, Bacillus cereus MSU AS was isolated from the gut of a marine fish Sardinella longiceps. In total, seven agricultural waste substrates (coconut oil cake, neem oil cake, onion skin waste, wheat bran, jack fruit waste, banana skin peels and pomegranate skin peel) were selected for solid state production of lipase by B. cereus MSU AS. Among the tested substrates, onion...

Bacillus cereus causes food poisoning and serious non-gastrointestinal-tract infections. Non-hemolytic enterotoxin (Nhe), which is present in most B. cereus strains, is considered to be one of the main virulence factors. However, a B. cereus ΔnheBC mutant strain lacking Nhe is still cytotoxic to intestinal epithelial cells. In a screen for additional cytotoxic factors using an in vitro model fo...

Pseudomonas chlororaphis PA23, Pseudomonas spp. strain DF41, and Bacillus amyloliquefaciens BS6 consistently inhibit infection of canola petals by Sclerotinia sclerotiorum in both greenhouse and field experiments. Bacillus thuringiensis BS8, Bacillus cereus L, and Bacillus mycoides S have shown significant inhibition against S. sclerotiorum on plate assays. The presence of antibiotic biosynthet...

materials and methods two-hundred pasteurized (n = 100) and nonpasteurized (n = 100) cheese samples were collected. the initial isolation was performed on pemba specific medium. antibiotic susceptibility testing was performed using several antibiotic disks, according to the guidelines of the clinical laboratory and standards institute. specific primers amplifying the hbla enterotoxin-encoding g...

Maari is a spontaneously alkaline fermented food condiment made from baobab tree seeds. Due to the spontaneous nature of maari fermentations growth of the opportunistic human pathogen Bacillus cereus is occasionally observed. Bacillus subtilis strains are important for alkaline seed fermentations because of their enzymatic activities contributing to desirable texture, flavor and pH development....

More evidence has recently been presented suggesting that Bacillus species other than Bacillus anthracis should not be readily discarded as contaminants, especially when isolated from neutropenic patients with cancer1•`3). Although very rare, infections with Bacillus cereus, such as bacteremia, pneumonia or meningitis, are most likely to occur in patients with acute leukemia, and often follow a...

Flagellar antigen of Bacillus cereus H.1 was purified and tested for serodiagnostic antigen by ELISA. The antibody against the flagellar antigen of B. cereus H.1 reacted not only with the homologous specific antigen but also reacted with the flagellar antigens of 23 strains of B. cereus. This common flagellar antigen of B. cereus was found to be due to 61-kDa protein by SDS-PAGE and immunoblot ...

The Bacillus cereus group contains vertebrate pathogens such as Bacillus anthracis and Bacillus cereus and the invertebrate pathogen Bacillus thuringiensis. Microbial biopesticides based on B. thuringiensis (Bt) are widely recognized as being among the safest and least environmentally damaging insecticidal products available. Nevertheless, a recent food poisoning incident prompted a European Fo...

Polyhydroxybutyrate producing bacteria from garden soil were isolated and characterized for their morphological, biochemical properties. Based on their 16S rRNA gene sequences, they were identified as Bacillus mycoides DFC1, Bacillus cereus DC1, Bacillus cereus DC2, Bacillus cereus DC3 and Bacillus cereus DC4. The bacteria were screened for PHB production and compared for the intensity of fluor...

In recent years Bacillus cereus has gained increasing importance as a food poisoning pathogen. It is the eponymous member of the B. cereus sensu lato group that consists of eight closely related species showing impressive diversity of their pathogenicity. The high variability of cytotoxicity and the complex regulatory network of enterotoxin expression have complicated efforts to predict the tox...