DNA metabarcoding has great potential to improve marine biomonitoring programs by providing a rapid and accurate assessment of species composition in zoobenthic communities. However, some methodological improvements are still required, especially regarding failed detections, primers efficiency incompleteness databases. Here we assessed the two different marker loci (COI 18S) three primer pairs ...

We describe PCR primers and amplification protocols developed to obtain introns from conserved nuclear genes in deep-sea protobranch bivalves. Because almost no sequence data for protobranchs are publically available, mollusk and other protostome sequences from GenBank were used to design degenerate primers, making these loci potentially useful in other invertebrate taxa. Amplification and sequ...

UNLABELLED PREMISE OF THE STUDY To complement existing sets of primarily dinucleotide microsatellite loci from noncoding sequences of date palm, we developed primers for tri- and hexanucleotide microsatellite loci identified within genes. Due to their conserved genomic locations, the primers should be useful in other palm taxa, and their utility was tested in seven other Phoenix species and ...

background: human granulocyte colony-stimulating factor (hg-csf) can serve as valuable biopharmaceutical for research and treatment of the human blood cancer. transplastomic plants have been emerged as a new and high potential candidate for production of recombinant biopharmaceutical proteins in comparison with transgenic plants due to extremely high level expression, biosafety and many other a...

The molecular characterization of chilli germplasm was done based on estimation genetic diversity among the by using SSR markers. Forty germplasms were analyzed eight primers. primers produced 30 loci with an average value 3.75 alleles per locus. similarity index matrix ranged from zero to 2.74. Polymorphic information content (PIC) 0.543 0.735 0.658. highest number (five) allele observed in pr...

one hundred and fifty blood samples were prepared from cattle of a region in isfahan. the extracted dna from blood cells were analyzed by a. centrale (amori strain) specific nested-pcr using primers derived from 16s rrna gene. all blood smears were negative for a. centrale like structures. the results showed that 2 of total 150 blood samples (1.33%) were a. centrale (amori strain) positive by s...

The allelic variation at seven prolamin loci involved in quality has been studied in a set of durum wheat landraces from all the Spanish regions where this crop has been traditionally cultivated. The genetic variability was higher than that found in other germplasm collections. All the loci, except Glu-B2, displayed a genetic variability higher than 0.62, with Glu-3 the most polymorphic. In tot...