A viable route has been developed for the selective synthesis of the 4→6-linked catechin dimers, scarcely accessible from Nature and/or through synthesis. An acyclic nucleophilic catechin precursor (seco-catechin) was used for the regioselective union with an electrophilic catechin unit, and subsequent pyran cyclization gave the desired 4→6-linked dimers, i.e., procyanidin B6 and catechin-(4α→6...

Evidence for the recognition of a secondary structure of carrageenan chains present in red algae tissues. Abstract We report the isolation, for the first time by phage display, of a scFv recombinant antibody called B3 directed against carrageenans, the major sulphated polysaccharides of red seaweeds. Immunoassays were used to characterize the binding of B3 antibodies towards the three main carr...

The amino acid sequence of Chromatium ferredoxin was determined by studies of thermolysin peptides of S-carboxymethylcysteinylferredoxin and chymotryptic peptides of oxidized ferredoxin. The 81 amino acid residues in the molecule have the following sequence: Ala-Leu-Met-IleThr-Asp-Gln-Cys-Ile-Asn-Cys-Asn-Val-Cys-Gln-ProGlu-Cys-Pro-Asn-Gly-Ala-Ile-Ser-Gln-Gly-Asp-Glu-ThrTyr-Val-Ile-Glu-Pro-Ser-L...

The transport and metabolism of glutamine (GLN) and glutamate (GLU) during fetal development exhibit unique characteristics that clearly emphasize the importance of the interaction between the placenta and the fetal liver. GLN is delivered into the fetal circulation at a rate that is the highest of all the amino acids. In contrast, approximately 90% of fetal plasma GLU is extracted by the place...

When purified under rigorous conditions, some murine anti-double-stranded-DNA (anti-dsDNA) antibodies actually bind chromatin rather than dsDNA. This suggests that they may actually be antinucleosome antibodies that only appear to bind dsDNA when they are incompletely dissociated from nucleosomes. Experiments in murine models suggest that antibody-nucleosome complexes may play a crucial role in...

Many cell types contain a subset of long-lived, 'stable' microtubules that differ from dynamic microtubules in that they are enriched in post-translationally detyrosinated tubulin (Glu-tubulin). Elevated Glu tubulin does not stabilize the microtubules and the mechanism for the stability of Glu microtubules is not known. We used detergent-extracted cell models to investigate the nature of Glu mi...

OBJECTIVE Previous studies have shown the importance of somatic mutations and arginine residues in the complementarity-determining regions (CDRs) of pathogenic anti-double-stranded DNA (anti-dsDNA) antibodies in human and murine lupus, and in studies of murine antibodies, a role of mutations at position 53 in V(H) CDR2 has been demonstrated. We previously demonstrated in vitro expression and mu...

The joint use of multi-frequency signals brings new prospects for precise positioning and has become a trend in Global Navigation Satellite System (GNSS) development. However, a new type of inter-frequency clock bias (IFCB), namely the difference between satellite clocks computed with different ionospheric-free carrier phase combinations, was noticed. Consequently, the B1/B3 precise point posit...