Cryptosporidium species are one of the most common causes of gastrointestinal infection in humans around the world. This study has aimed to investigate the hyper variable region of the 18S rRNA gene in Cryptosporidium for exact parasite identification .   DNA was extracted from 26 fecal samples from which initially Cryptosporidium oocysts were identified by Ziehl-Neelsen acid-fast , Auramine ...

background: cryptosporidiosis is a common coccidian parasite infection in patients with diarrhea that has worldwide distribution especially in developed countries. therefore,the aim of this study was to determine the occurrence of cryptosporidium infection in patients with gastroenteritis admitted to hospitals of mazandaran university of medical sciences by parasitological and molecular methods...

cryptosporidium is an obligate intracellular protozoan parasite infecting a wide range of hosts. the current study investigated the genetic profile of cryptosporidium species in calves in liverpool, england. fifty-two calve fecal samples were collected from a farm and initially screened by auramine phenol, modified ziehl-neelsen and elisa. pcr analysis of 18s rrna gene was carried out for the p...

background: tuberculosis (tb) is an increasing public health concern worldwide. on a global scale it has a devastating impact in developing nations. genital tb, an extrapulmonary form, is not uncommon particularly in areas where pulmonary tb is prevalent. genital tb may be asymptomatic or may even masquerade as other gynaecological conditions; hence, diagnosis requires a high degree of suspicio...

In this study, the elucidation of the toxicity mechanism was undertaken regarding the IC(50) of the oxygen uptake rate (OUR) with relevance to the biosorption as a toxicity evaluation of chemical substances for activated sludge (AS). At the IC(50) of<100 mg/l, malachite green (MG) and crystal violet (CV) were confirmed in the group showing relatively strong OUR inhibition. These dyes were marke...

In an earlier paper from this laboratory (1) there were described the characteristics of competitive inhibition resulting from the interference of certain aromatic compounds with the association of flavin adenine dinucleotide and the separated protein of n-amino acid oxidase. Included among the inhibitors were auramine, quinine, and various other quinolines and anilines. We now present our find...

BACKGROUND It is difficult to determine whether early tuberculosis treatment is effective in reducing the infectiousness of patients' sputum, because culture takes weeks and conventional acid-fast sputum microscopy and molecular tests cannot differentiate live from dead tuberculosis. METHODS To assess treatment response, sputum samples (n=124) from unselected patients (n=35) with sputum micro...

BACKGROUND "TBDx" is an innovative smear microscopy system that automatically loads slides onto a microscope, focuses and digitally captures images and then classifies smears as positive or negative using computerised algorithms. OBJECTIVES To determine the diagnostic accuracy of TBDx, using culture as the gold standard, and compare this to a microscopist's diagnostic performance. METHODS T...

Although most mycobacterial lipids are thought to be associated with the cell envelope, the authors previously observed substantial deposits of intracellular lipophilic material. A Nile-red-based cytological assay was used to determine factors which affect the presence and natural history of intracellular lipophilic inclusions (ILIs) in Mycobacterium smegmatis. Development of ILIs was associate...