After characterization of the porin OmpF and selection of molecular structures responsible for leukocyte activation by using computer-assisted epitope analysis, the analogs OmpF (153-174) (containing amino acids 153 to 174), OmpF (157-174), and OmpF (275-285) were synthesized and tested. Like the native protein, the segments were mitogenic for BALB/c splenocytes and induced B lymphocyte differe...

We report the analysis and characterization of natural and modified oligonucleotides by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The present technology was highly improved for this class of compounds by using a new matrix, 2,4,6-trihydroxy acetophenone, together with di- and triammonium salts of organic or inorganic acids to suppress peak broa...

A novel uronic acid-containing glycosphingolipid (UGL-1) was isolated from the ascidian Halocynthia roretzi. UGL-1 was prepared from chloroform-methanol extracts and purified by the use of successive column chromatography on DEAE-Sephadex, Florisil, and Iatrobeads. Chemical structural analysis was performed using methylation analysis, gas chromatography, gas chromatography-mass spectrometry, ma...

Boronic acids have been widely used in a wide range of organic reactions, in the preparation of sensors for carbohydrates, and as potential pharmaceutical agents. With the growing importance of click reactions, inevitably they are also applied to the synthesis of compounds containing the boronic acid moiety. However, such applications have unique problems. Chief among them is the issue of coppe...

Background and Aims The effect of amino acids, their interactions with volatiles other non-volatiles, on in-mouth sensory properties red wines is not known. This knowledge gap has been studied in a series comprehensive experiments. Methods Results A solvent-assisted flavour evaporation extract Shiraz wine volatiles, de-aromatised polyphenolic acids were added to model systems. Using full factor...

Purpose: We conducted a study that examined the physicochemical properties of Eriobotrya japonica leaf enzyme-assisted extract (EJLE) from Jeju Island.Methods: EJLE was prepared using pepsin enzyme. The antioxidant and antiaging effects were investigated to determine its utility as functional material.Results: In analysis total amino acids, 16 kinds components isolated EJLE. DPPH ABTS radical s...

Polyphenols, as plant secondary metabolites, achieve strong antioxidant capacity by scavenging free radicals, chelating metals, and interacting with lipid membranes, proteins nucleic acids. The aim of the present study was examination applied extraction conditions (particle size material, solvent-to-solid ratio, solvent type, time) techniques (maceration, heat- ultrasound-assisted extractions) ...

Pectin is one of the most widely used functional polysaccharide, which extensively in food, biotechnological, chemical, cosmetic, and pharmaceutical industries. can be suitably extracted from apple pomace main by-product juice industry. In this study, pectin obtained microwave assisted extraction (MAE) conventional (CE) were compared terms yield, time temperature. The type acids namely organic ...

Pollen provides floral visitors with essential nutrients including proteins, lipids, vitamins and minerals. As an important nutrient resource for pollinators, including honeybees and bumblebees, pollen quality is of growing interest in assessing available nutrition to foraging bees. To date, quantifying the protein-bound amino acids in pollen has been difficult and methods rely on large amounts...