β-Fructosidases are a widespread group of enzymes that catalyze the hydrolysis of terminal fructosyl units from various substrates. These enzymes also exhibit transglycosylation activity when they function with high concentrations of sucrose, which is used to synthesize fructooligosaccharides (FOS) in the food industry. A β-fructosidase (BfrA) with high transglycosylation activity was purified ...

The method uses the principal component analysis and multidimensional scaling method to classify the sequences of tRNA genes into multiple groups of similar sequences, and also to extract characteristic bases that are conserved within a group but differ from other groups. This procedure was applied recursively to classify the sequences into hierarchical groups so that characteristic sites can b...

The single-strand-specific endonuclease S1 from Aspergillus oryzae introduces highly selective cleavages into supercoiled covalently closed circular DNA molecules, but not into their previously linearized counterparts. The cleavage sites are inverted repeats of unit length between 9 and 13 base pairs, separated by a nonrepetitious 2-6 base pairs. Such regions may adopt hairpin or similar struct...

Daily infusion of brinase (Protease 1 of Aspergillus oryzae) for 10 to 30 days in six patients (three children, three adults) with acute leukemia resulted in the production of complement-dependent autocytotoxicity against leukemic cells and lymphocytes and, in some instances, against platelets. This appeared to be due to the production of autoantibody, but its nature remains to be elucidated. T...

The present study deals the optimization of cultural conditions for the production of alpha amylase by Aspergillus oryzae IIB-30 and its mutant derivative A. oryzae EMS-18 in stirred fermenter. The time of fermentation for enzyme production by both wild and mutant strains was studied. It was found that the time required for maximal enzyme production (608 U/ml) in case of mutant strain was reduc...

We report here a development of the MultiSite Gateway(TM)-based versatile plasmid construction system applicable for the rapid and efficient preparation of Aspergillus oryzae expression plasmids. This system allows the simultaneous connection of the three DNA fragments inserted in entry clones along with a destination vector in a defined order and orientation. We prepared a variety of entry clo...

cr(iii), co(ii), ni(ii), cu(ii) and zn(ii) salts of 2-(4-nitrophenylaminocarbonyl)benzoic acid were characterized by physical, analytical and spectroscopic studies and checked for their  in-vitro antimicrobial activity against three bacterial strains, mycobacterium smegmatis (gram +ve), escherichia coli (gram -ve), pseudomonas aeuroginosa (gram -ve) and three fungal strains, nigrospora oryzae, ...

Alpha amylase (α-amylase) enzyme is used as a thinning agent in the starch hydrolysis and it is widely used in the food processing, paper and textile industries. The intention of the present study was to analyse the α-amylase activity in solid state fermentation and submerged fermentation using immobilized cells of Aspergillus oryzae (A. oryzae) and Bacillus cereus (B. cereus), respectively. Th...

The koji mold Aspergillus oryzae secretes a prolyl dipeptidyl peptidase (DPPIV) when the fungus is cultivated in a medium containing wheat gluten as the sole nitrogen and carbon source (MMWG). We cloned and sequenced the DPPIV gene from an A. oryzae library by using the A. fumigatus dppIV gene as a probe. Reverse transcriptase PCR experiments showed that the A. oryzae dppIV gene consists of two...

We constructed a protein expression vector with an improved enoA promoter that harbored 12 tandem repeats of the cis-acting element (region III) of Aspergillus oryzae. The improved promoter yielded reporter beta-glucuronidase (GUS) activity approximately 30-fold of the original promoter. Northern blot analysis confirmed that GUS expression was increased at the transcriptional level. The transfo...