Extracts of frozen rat liver were found to catalyse the formation of 3H2O from DL-2-hydroxy[2-3H]glutarate. Three peaks of enzyme activities were observed on separation by chromatography on DEAE-Sepharose. The first and second peaks corresponded to an enzyme acting on L-2-hydroxyglutarate and the third peak corresponded to an enzyme acting on D-2-hydroxyglutarate, as indicated by competitive in...

Enzymes catalyze various biochemical functions with high efficiency and specificity. In vitro design of the enzyme leads to novel bioactivity in this natural biomolecule that give answers of some vital questions like crucial residues in binding with substrate, molecular evolution, cofactor specificity etc. Enzyme engineering technology involves directed evolution, rational designing, semi-ratio...

Inefficient CO(2) removal due to limited diffusion represents a significant barrier in the development of artificial lungs and respiratory assist devices, which use hollow fiber membranes (HFMs) as the blood-gas interface and can require large blood-contacting membrane area. To offset the underlying diffusional challenge, "bioactive" HFMs that facilitate CO(2) diffusion were prepared via covale...

The size of the mutant N-acetylglucosamine 1-phosphotransferase in Golgi membranes from fibroblasts of patients with I-cell disease and classical pseudo-Hurler polydystrophy, which comprised one complementation group characterized by deficiency towards both artificial and natural acceptor substrates, was significantly smaller than the normal enzyme, 151-174 kDa compared with 225-278 kDa. The si...

The production of chemicals from renewable resources is gaining importance in the light of limited fossil resources. One promising alternative to widespread fermentation based methods used here is Synthetic Cascade Biomanufacturing, the application of minimized biocatalytic reaction cascades in cell free processes. One recent example is the development of the phosphorylation independent convers...

A malate oxidase which uses L-malate and FAD as substrates is produced in mutant strains of Escherichia coli lacking the activity of NAD-dependent malate dehydrogenase. Expression of the oxidase is independent of the presence or absence in the mutants of a material cross-reacting with antibodies against malate dehydrogenase. It is surmised that products of malate dehydrogenase are responsible f...

Proper preservation of transgenes and transgenic materials is important for wider use of transgenic technology in plants. Here, we report stable preservation and faithful expression of a transgene via artificial seed technology in alfalfa. DNA constructs containing the uid reporter gene coding for β-glucuronidase (GUS) driven by a 35S promoter or a tCUP promoter were introduced into alfalfa via...

Context The development and survival of floodplain forests are dependent on the occurrence seasonal flooding that provides soil moisture nutrient availability suitable for microorganisms, which main sources enzymes involved in decomposition mineralisation organic matter. Aims We compared enzyme activities a forest Lower Vistula Valley, Poland, cut off 80 years from river by artificial embankmen...

Online Publication Date: 01 June 2007 To cite this Article: De Meo, Pasquale, Rosaci, Domenico, Sarnè, Giuseppe M.L., Ursino, Domenico and Terracina, Giorgio , (2007) 'EC-XAMAS: SUPPORTING E-COMMERCE ACTIVITIES BY AN XML-BASED ADAPTIVE MULTI-AGENT SYSTEM', Applied Artificial Intelligence, 21:6, 529 562 To link to this article: DOI: 10.1080/08839510701409052 URL: http://dx.doi.org/10.1080/088395...