The integrity of epithelial and endothelial barriers in the lower airspaces of the lungs has to be tightly regulated, in order to prevent leakage and to assure efficient gas exchange between the alveoli and capillaries. Both G(-) and G(+) bacterial toxins, such as lipopolysaccharide and pneumolysin, respectively, can be released in high concentrations within the pulmonary compartments upon anti...

OBJECTIVES Intraperitoneal (IP) injection of 3.5 g/kg L-arginine (known to induce acute pancreatitis) in rats will result in much greater increases in serum ornithine versus citrulline concentration (Crit Care Med. 2008;36:2117-2127). These data indicate a major role of arginase in the catabolism of L-arginine. Therefore, we tested the effects of the irreversible arginase inhibitor (+)-S-2-amin...

The breakdown of L-arginine to ornithine and urea by host arginase supports Leishmania proliferation in macrophages. Studies using arginase-null mutants show that Leishmania-derived arginase plays an important role in disease pathogenesis. We investigated the role of parasite-derived arginase in secondary (memory) anti-Leishmania immunity in the resistant C57BL/6 mice. We found that C57BL/6 mic...

Arginase is a metalloenzyme which hydrolyzes L-arginine to L-ornithine and urea. Since its discovery, in the early 1900s, this enzyme has gained increasing attention, as literature reports have progressively pointed to its critical participation in regulating nitric oxide bioavailability. Indeed, accumulating evidence in the following years would picture arginase as a key player in vascular hea...

A two-stage study was carried out to test the mechanism of arginase in ammonia detoxification yellow catfish. At stage 1, fish injected lethal half concentration ammonium acetate and 0.9% sodium chloride respectively every 12 h six replicates for 72 h. The result found that no significant different serum contents group at hours 12, 24, 36, 48, 60 72. 2, split two, one continued with (NH3 group)...

: Hepatocellular carcinoma (HCC) is the most prevalent type of primary cancer liver in adults represent about 80%-90% all cancers. It essential to differentiate HCC and intrahepatic cholangiocarcinoma metastatic carcinoma. Arginase-1 was considered as sensitive specific marker benign malignant hepatocyte. This study aimed detect diagnostic role immunohistochemical expression arginase-1 differen...

in the present study, under typical management conditions of dairy farms in iran, the effect of oral administration of on serum glucose, ast and arginase(indices of liver injuries) of oral monensin was assessed. thirteen multiparous holstein cows were blocked into a control and an experiment group from 3 weeks prepartum to 45 days postpartum according the number of their calvings. the groups re...

ILCs are an emerging family of innate immune cells that exhibit phenotypic and functional heterogeneity that is remarkably similar to effector subsets of CD4 Th cells [1, 2]. As such, ILCs can be placed into three groups based on expression of cytokines, cytokine receptors, and transcription factors, including T-bet Group 1 ILCs that are comparable with Th1 cells, GATA3 Group 2 ILCs that are co...

l-Arginine metabolism by the arginase and nitric oxide (NO) synthase (NOS) families of enzymes is important in NO production, and imbalances between these pathways contribute to airway hyperresponsiveness (AHR) in asthma. To investigate the role of arginase isozymes (ARG1 and ARG2) in AHR, we determined the protein expression of ARG1, ARG2, the NOS isozymes, and other proteins involved in l-arg...

We have previously reported arginase expression in human breast cancer cells and demonstrated that the inhibition of arginase by N(ω) hydroxy L-arginine (NOHA) in MDA-MB-468 cells induces apoptosis. However, arginase expression and its possible molecular targets in human breast tumor samples and potential clinical implications have not been fully elucidated. Here, we demonstrate arginase expres...