During development, coordinated cell shape changes alter tissue shape. In the Drosophila ventral furrow and other epithelia, apical constriction of hundreds of epithelial cells folds the tissue. Genes in the Gα12/13 pathway coordinate collective apical constriction, but the mechanism of coordination is poorly understood. Coupling live-cell imaging with a computational approach to identify contr...

We have investigated the relationships between the apical sorting mechanism using lipid rafts and the soluble N-ethyl maleimide-sensitive factor attachment protein receptor (SNARE) machinery, which is involved in membrane docking and fusion. We first confirmed that anti-alpha-SNAP antibodies inhibit the apical pathway in Madin- Darby canine kidney (MDCK) cells; in addition, we report that a rec...

This study examined perception of multiple coronal places of articulation by native listeners of Western Arrernte. Three main findings emerged. 1) Coronal nasals and laterals are as perceptually robust as coronal stops. 2) Across manners of articulation, apical alveolars are less perceptually robust than other coronals. 3) Formant transitions from a preceding vowel are necessary to correctly id...

Identification and surgical closure of apical muscular ventricular septal defects are associated with certain morbidity and mortality because of their location in the ventricular septum distal to the moderator band, making adequate visualization and complete closure from the right atrium almost impossible. However, the precise margins of apical VSDs can be readily identified by a small apical r...

We studied the sorting and surface delivery of three apical and three basolateral proteins in the polarized epithelial cell line Caco-2, using pulse-chase radiolabeling and surface domain-selective biotinylation (Le Bivic, A., F. X. Real, and E. Rodriguez-Boulan. 1989. Proc. Natl. Acad. Sci. USA. 86:9313-9317). While the basolateral proteins (antigen 525, HLA-I, and transferrin receptor) were t...

Polarized epithelial cells develop and maintain distinct apical and basolateral surface domains despite a continuous flux of membranes between these domains. The Na(+)/H(+)exchanger NHE6 localizes to endosomes but its function is unknown. Here, we demonstrate that polarized hepatoma HepG2 cells express an NHE6.1 variant that localizes to recycling endosomes and colocalizes with transcytosing bu...

objective: one of the principle purposes of root canal obturation is to obtain hermetic sealing of the root canal system. according to the development of technology, many materials are now used in root canal filling. an in vitro dye leakage study was performed to evaluate the apical sealability of white-colored mineral trioxide aggregate (wmta) and gray-colored portland cement (gpc) when used a...