In this work, we obtain a producer of the recombinant SARS-CoV-2 E protein and develop system for its expression purification in soluble form. ELISA confirmed antigenic properties. COVID-19 convalescents, level IgG against was significantly increased comparison with control group patients.

A strategy is presented that allows a causal analysis of co-expressed genes, which may be subject to common regulatory influences. A state-of-the-art promoter analysis for potential transcription factor (TF) binding sites in combination with a knowledge-based analysis of the upstream pathway that control the activity of these TFs is shown to lead to hypothetical master regulators. This strategy...

Research Theme 1. Quantification of trace amount of proteins (Etsuro Ito, Mika Morikawa, Mugiho Kaneda) We are developing the method to detect and quantify very tiny amount of a given protein in a small volume, such as in a single cell. We aim at establishing an enterprise to commercialize protein quantification reagents and kits based on this technique. Using our “enzyme cycling method”, it is...

[1] We present a novel explanation of the physical processes behind one type of cloud and ground-level tornadogenesis within a supercell. We point out that the charge separation naturally found in these large thunderstorms can potentially serve to contract the preexisting angular momentum through the additional process of the electric force. On the basis of this, we present a plausible geometry...

Homomorphic signatures (HS) allows the derivation of the signature of the message-function pair (m, g), where m = g(m1, . . . ,mK), given the signatures of each of the input messages mk signed under the same key. Multi-key HS (M-HS) introduced by Fiore et al. (ASIACRYPT’16) further enhances the utility by allowing evaluation of signatures under different keys. While the unforgeability of existi...

Recent developments in voting theory show that Condorcet profiles embedded in electorates are responsible for conflicts between pairwise voting methods and for reversals of rankings under positional methods whenever candidates are dropped or added. Because of the strong symmetry of the rankings of the candidates within these profiles, it can be argued that Condorcet profiles represent complete ...

Quantifying and predicting the antigenic characteristics of a virus is something of a holy grail for infectious disease research because of its central importance to the emergence of new strains, the severity of outbreaks, and vaccine selection. However, these characteristics are defined by a complex interplay of viral and host factors so that phylogenetic measures of viral similarity are often...

Subacute sclerosing panencephalitis (SSPE) is a progressive, lethal complication of measles caused by particular mutants of measles virus (MeV) that persist in the brain despite high levels of neutralizing antibodies. We addressed the hypothesis that antigenic drift is involved in the pathogenetic mechanism of SSPE by analyzing antigenic alterations in the MeV envelope hemagglutinin protein (Me...