2 Platelet α-granules constitute the major rapidly releasable reservoir of thrombospondin-1 in higher animals. Although some fragments and peptides derived from thrombospondin-1 stimulate or inhibit platelet aggregation, its physiological function in platelets has remained elusive. We now show that endogenous thrombospondin-1 is necessary for platelet aggregation in vitro in the presence of phy...

Impaired healing is common in wounds infected with the major human pathogen Staphylococcus aureus, although the underlying mechanisms are poorly understood. Here, we show that S. aureus lipoteichoic acid (LTA) inhibits platelet aggregation caused by physiological agonists and S. aureus and reduced platelet thrombus formation in vitro. The presence of D-alanine on LTA is necessary for the full i...

Nitric oxide and other reactive oxygen species generated by nitric-oxide synthases (NOS) modulate, among several other cellular responses, the production of eicosanoids and platelet aggregation. The roles of specific NOS in these two phenomena remain to be determined. Thus, the present study assessed whether inducible NOS (iNOS) and endothelial NOS (eNOS) modulate in a similar manner the produc...

ABSTFACT Prostaglandin E (PGE1) sensitive adenylate cyclase rEC.4.6.1.1 ATP-pyrophosphaie lyase (cyclizing)] in human platelet lysates is inhibited by dextran sulfate. Inhibition is dose-dependent at the range of l-10 ug/ml. Maximal inhibition obtained was 85% at 30 ug/ml . At a similar range of concentrations dextran sulfate was found to antagonize the anti-aggregating effect of PGEl on adenos...

In our present investigation, a series of novel 4-methoxy-1,3-benzenediolyl-hydrazones were designed and synthesized, and their ability to inhibit platelet aggregation was evaluated by adenosine diphosphate (ADP) and arachidonic acid (AA). The structures of the synthesized compounds were confirmed by spectral data. Results demonstrated that the activities of all compounds excelled the positive d...

FAK is a focal adhesion kinase that is phosphorylated on tyrosine in activated platelets. Induction of FAK phosphorylation requires both fibrinogen binding to integrin alpha IIb beta 3 and post-occupancy events during agonist-induced platelet aggregation or platelet spreading on a fibrinogen matrix. To identify the signaling pathways necessary for tyrosine phosphorylation of FAK, we have examin...

Within the present study, effects of fermentation on anti-inflammatory and anti-platelet properties both homemade commercially purchased bovine dairy almond, coconut, rice-based alternatives were evaluated. The extracted total lipids (TL) from fermented unfermented bovine, products further separated into their neutral (NL) polar (PL) fractions by counter current distribution. TL, PL, NL each sa...