In the present study, the feeding stage, body parts, development and sex specific activity of Glutathione S-transferases (GSTs) were observed in different mosquito species (Aedes aegypti, Culex quinquefasciatus, Anopheles stephensi, An. culicifacies, An. annularis, An. subpictus, An. vagus). GST activity was assayed spectrophotometrically at 23°C, using a UV Max microplate Reader, to measure th...

b a ckground: the long-lasing insecticide impregnated nets (llins) is considered as an effective tools for malaria vector control. the aim of this study was to evaluate the residual efficacy of alpha-cypermethrin long lasting im- pregnated nets (llin-interceptor®) against anopheles stephensi using tunnel test. m e t hods: the wash-resistance of interceptor® nets were assessed under laboratory c...

Mammalian herbivores, particularly dietary specialists must have an efficient means to metabolize the high doses of plant secondary compounds they consume. We found previously that Neotoma stephensi, a juniper specialist, upregulated catechol-O-methyl transferase (COMT) mRNA almost seven fold in response to an ecologically relevant diet (70% juniper). To further investigate the relevance of thi...

BACKGROUND & OBJECTIVES Two bacterial larvicide (bio-larvicide) formulations--Bacticide and VectoBac containing viable endospores and delta endotoxin of Bacillus thuringiensis var israelensis H-14 were evaluated in 2001 for their mosquito larvicidal efficacy under the operational conditions of urban malaria control programme in Surat city, India. METHODS Larvicides were applied at the recomme...

lectins that agglutinate red blood cells (rbcs) were demonstrated in anopheles stephensi mosquito midgut extracts using human (four groups: a, b, ab and o, rh+) rat, sheep and rabbit blood cells. only rabbit rbcs showed agglutination reaction against the midgut extracts. significant differences in hemagglutinin titers and carbohydrate specifity were detected between male and female mosquitoes a...

The introduction of exogenous genes into the genomes of mosquitoes requires microinjection techniques tailored to the specific species of interest. This video protocol demonstrates a method used by the James laboratory to microinject DNA constructs into Anopheles stephensi embryos for the generation of transformed mosquitoes. Techniques for preparing microinjection needles, collecting and prepa...

An earlier in vitro screening of N,N-diethyl-3-methylbenzamide (Deet) and N,N-diethylphenylacetamide (DEPA) analogs showed that two DEPA analogs, N,N-diethyl(3-bromophenyl) acetamide and N,N-diethyl[(alpha,alpha,alpha-trifluoro-m-tolyl)]acetamide, and one Deet analog, N,N,diethyl[3-(trifluoromethyl)]benzamide, had biting-deterrent activities that were superior to Deet against Aedes aegypti (L.)...

Transposon-based forward and reverse genetic technologies will contribute greatly to ongoing efforts to study mosquito functional genomics. A piggyBac transposon-based enhancer-trap system was developed that functions efficiently in the human malaria vector, Anopheles stephensi. The system consists of six transgenic lines of Anopheles stephensi, each with a single piggyBac-Gal4 element in a uni...

      Tagetes minuta L. is considered as an annual plant growing in the temperate zones of South America. It has been used as carminative, anti-inflammatory, anti-abortion,vermifuge, bronchodilator and hypotensive. In the current study the locally cultured plant of Tagetes minuta L. was studied for phytochemical and larvicidal properties.The phytochemical analysis of aerial pa...