نتایج جستجو برای: and 4 extender containing 15 mgml bsa bsa
تعداد نتایج: 17110129 فیلتر نتایج به سال:
BSA is unfolded by the surfactant CPB and is complexed with Tb (Tb–BSA–CPB). At the excitation wavelength of 290 nm, both BSA and CPB get excited. The CPB emission, which is comparatively blue shifted (310 nm) than that of BSA (340 nm), overlaps with the absorption band of BSA resulting in the energy transfer from CPB to BSA. BSA transfers its energy to Tb, which emits at 490 and 545 nm. When G...
It has been demonstrated that proteins covalently conjugated to folic acid may be taken up by cells via endocytosis after binding to a folate binding protein (FBP) in the cell membrane. The proteins taken up in this manner remain catalytically active and they may modify physiological processes occurring in the cytosol. Confocal fluorescence microscopy of KB cells incubated with FITC-bovine seru...
Recent studies demonstrated that endoplasmic reticulum (ER) stress regulates glucose homeostasis and that ER stress preconditioning which induces an adaptive, protective unfolded protein response (UPR) offers cytoprotection against nephrotoxins. Thus the aim of the present study was to use renal proximal tubule cells (PTCs) to further elucidate the link between the BSA-induced ER stress and alp...
Body surface area (BSA) has been used in clinical practice to determine dosage of medication and in standardisation of biological parameters such as creatinine clearance, cardiac out put etc. BSA is estimated from measured height and weight, either with a nomogram or using a mathematical equation. There are many mathematical equations in the published literature but many are complex and may not...
This paper investigates the bioseparation of binary protein mixtures using polystyrene based anion exchange resin. Adsorption experiments were conducted in batch mode using draft-tube internally recirculate dair lift biocontactor in comparison with the conventional shake flask batch adsorption equilibrium experiments. Binary protein mixtures contained bovine serum albumin (BSA) and bovine haemo...
Amine-functionalized mesoporous SBA-15 silica loaded with bovine serum albumin (BSA) has been successfully encapsulated with a thin layer coating of poly(acrylic acid) PAA, with the entrapped BSA being released from the PAA-encapsulated SBA-15 at the higher pH value of 7.4 rather than at the lower pH value of 1.2. This novel drug delivery system has a potential application in the release of pro...
Rosmarinic acid (RA) is an importantly and naturally occurring polyphenol from plants of the mint family with potent biological activities. Here, the in vitro interaction of RA with bovine serum albumin (BSA) has been investigated using various biophysical approaches as well as molecular modeling methods, to ascertain its binding mechanism and conformational changes. The fluorescence results de...
Progesterone conjugated to bovine serum albumin (BSA) was used as a probe to study sex differences and the effects of hormonal status on binding of progesterone to crude synaptosomal membrane preparations (P2) derived from the mediobasal hypothalamic-anterior hypothalamic-preoptic area or the corpus striatum. Binding of 125I-labeled BSA linked to progesterone at the 11 position of the steroid (...
BACKGROUND Glucose-induced protein glycation has been implicated in the progression of diabetic complications and age-related diseases. The anti-glycation potential of polyphenol-rich plant extracts has been shown previously. Bunium Persicum has been demonstrated to possess a high level of polyphenols. The aim of current in vitro study was to determine the possible inhibitory effect of Bunium P...
BACKGROUND The Pediatric Heart Network's Single Ventricle Reconstruction (SVR) trial randomized infants with single right ventricles (RVs) undergoing a Norwood procedure to a modified Blalock-Taussig or RV-to-pulmonary artery shunt. This report compares RV parameters in the 2 groups using 3-dimensional echocardiography. METHODS AND RESULTS Three-dimensional echocardiography studies were obtai...
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