We have constructed a large fosmid library from a mesophilic anaerobic digester and explored its 16S rDNA diversity using a high-density filter DNA-DNA hybridization procedure. We identified a group of 16S rDNA sequences forming a new bacterial lineage named WWE3 (Waste Water of Evry 3). Only one sequence from the public databases shares a sequence identity above 80% with the WWE3 group which h...

Distinct layers of iron(III) and manganese(IV) (Fe/Mn) oxides are found buried within the reducing part of the sediments in Lake Baikal and cause considerable complexity and steep vertical gradients with respect to the redox sequence. For the on-site investigation of the responsible biogeochemical processes, we applied filter tube samplers for the extraction of sediment porewater combined with ...

This study, sponsored by the Water Environment Research Foundation, focused on innovative methods for the capture of metals from stormwater. The two areas of focus were media filters and swales. This paper will focus on the filter studies. Twelve media were chosen for initial evaluation. Equilibrium and kinetic studies were formed on these media to assess their performance in capturing metals f...

A University of Cape Town (UCT) pilot plant combining both membrane bioreactor (MBR) and moving bed biofilm reactor (MBBR) technology was monitored. Three experimental Phases were carried out by varying the mixed liquor sludge retention time (SRT) (indefinite, 30 and 15 days, respectively). The system performance has been investigated during experiments in terms of: organic carbon, nitrogen and...

Portions of the 16S RNA from a urease-positive Bilophila wadsworthia strain were sequenced, and a probe was constructed. The probe was end labeled with [32P]ATP and polynucleotide kinase and hybridized on a nylon filter (by dot blot hybridization) to the immobilized rRNA of 12 B. wadsworthia strains and eight other anaerobic isolates. The probe efficiently hybridized only to the Bilophila strai...

This study investigated the microbial community in a full scale anaerobic baffled reactor and sequencing batch reactor system for oil-produced water treatment in summer and winter. The community structures of fungi and bacteria were analyzed through polymerase chain reaction–denaturing gradient gel electrophoresis and Illumina high-throughput sequencing, respectively. Chemical oxygen demand eff...

This research evaluated the efficiency of combined anaerobic-aerobic processes for the treatment of slaughterhouse wastewater. The anaerobic reactor consists of a 3.95 L Plexiglas column with 60 mm diameter and 140 cm height. The cylindrical particles of polyvinyl chloride with 2 mm diameter and 1250 kg m-3 density packed to 60 cm of column were used as biomass saving material. The d...