نتایج جستجو برای: amplified

تعداد نتایج: 36991  

2016
Rita Costa Graça Pereira Inmaculada Garrido Manuel María Tavares-de-Sousa Francisco Espinosa

Three different DNA-based techniques, Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR) and Amplified Fragment Length Polymorphism (AFLP) markers, were used for fingerprinting Dactylis glomerata genotypes and for detecting genetic variation between the three different subspecies. In this study, RAPD assays produced 97 bands, of which 40 were polymorphic (41.2%). The I...

Journal: :Mycological research 2006
Ramasamy Perumal Thomas Isakeit Monica Menz Seriba Katile Eun-Gyu No Clint W Magill

Sorghum downy mildew, caused by the obligate oomycete Peronosclerospora sorghi, has been controlled through the use of resistant cultivars and seed treatment with metalaxyl. A recent outbreak in fields planted with treated seed revealed the presence of a metalaxyl-resistant variant. Here, PCR-based methods including amplification from RAPD primers and two systems of automated AFLP analysis have...

Journal: :Genetics and molecular research : GMR 2010
S Nawaz F A Khan S Tabasum M Zakria A Saeed M Z Iqbal

Forty sugarcane genotypes (clones), including elite lines, commercial cultivars of Saccharum officinarum and S. barberi clones, were fingerprinted with 30 RAPD markers, using a PCR-based marker assay. The genetic distance for RAPD data was determined according to Nei, and relationships between accessions were graphed in a dendrogram. Genetic distance values ranging from 16.2 to 86.3% were obser...

2015
Ambika B Gaikwad

RAPD is a PCR based technique which involves the use of single arbitrary. Advantages disadvantages, limitations and applications of RAPD.RAPD is a modification of the PCR in which a single, short and arbitrary. Therefore, RAPD technique can be performed in a moderate laboratory for most of.sophisticated equipment has made the RAPD technique valuable although. Tartflmann odak noktas ise de, RAPD...

Journal: :Infectious Diseases in Obstetrics and Gynecology 1997
Jacques F. G. M. Meis Harry L. Muytjens Paul P. van den Berg Andreas Voss Willem J. G. Melchers

OBJECTIVE Streptococcus pyogenes is the cause of the classical childbed fever and can occur in both sporadic and epidemic form. Once an outbreak is identified on a maternity ward it is not only necessary to place the patients in strict isolation but also identify to the source of the infection. Fast reliable typing methods can aid in infection control. METHODS An outbreak of puerperal fever d...

Journal: :Genetics and molecular research : GMR 2010
E Orhan S Ercisli

Mulberry (Morus spp, Moraceae) is an important horticultural crop in Turkey, which is one of the main world producers of mulberry fruit. We evaluated the genetic relationships among 26 mulberry genotypes selected for agronomic characteristics, using RAPD markers. A total of 367 DNA markers were generated with 34 random primers. The highest genetic similarity (0.80) was observed between Oltu58 (...

Journal: :iranian journal of public health 0
k diba dept. of parasitology and mycology, school of public health and institute of public health research sh mirhendi dept. of parasitology and mycology, school of public health and institute of public health research p kordbacheh dept. of parasitology and mycology, school of public health and institute of public health research n jalalizand laboratory of molecular biology, esfehan research center of public health, iran

background: aspergillus species are associated with allergic bronchopulmonary disease, mycotic keratitis, otomycosis, na­sal sinusitis and invasive infection. in this study, we developed a pcr-single strand confomational polymorphism method to identify the most common aspergillus species and we showed some advantages of this method comparing a pcr-restric­tion fragment length polymorphism with ...

Journal: :Mycological research 2003
Ronald T Kothera Anthony P Keinath Ralph A Dean Mark W Farnham

Didymella bryoniae (anamorph Phoma cucurbitacearum) is an ascomycete that causes gummy stem blight, a foliar disease that occurs on cucurbits in greenhouses and fields throughout the world. In a previous study using RAPD analysis, little genetic diversity was found among isolates of D. bryoniae from New York and South Carolina, USA. Here we report the use of amplified fragment length polymorphi...

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