Abstract Light‐oxygen‐voltage (LOV) domains are widespread photosensory modules that can be used in fluorescence microscopy, optogenetics and controlled production of reactive oxygen species. All the currently known LOV have absorption maxima range ~440 to ~450 nm, it is not clear whether they shifted significantly using mutations. Here, we generated a panel domain variants by mutating key chro...

UNLABELLED The web application oriented on identification and visualization of protein regions encoded by exons is presented. The Exon Visualiser can be used for visualisation on different levels of protein structure: at the primary (sequence) level and secondary structures level, as well as at the level of tertiary protein structure. The programme is suitable for processing data for all genes ...

The binding of lactose repressor to poly d(A-T) at low ionic strength has been investigated by heat denaturation. The poly d(A-T) melting is monitored by optical density and the protein melting by circular dichroism. From the modification of the poly d(A-T) melting curve we estimate a maximum binding ratio of about one tetrameric repressor to about 20 basic pairs. The repressor melting can be i...

ABSTRACT Antibody therapies have become an important component in the management of malignant disease. Antibodies can block tumour growth factors or their receptors, activate immunological attack on the tumour, and are used to deliver payloads such as radioisotopes, cytotoxic drugs or toxins. Immunotoxins are a new class of antitumour agents consisting of tumour- selective ligands (generally mo...

Homocysteine is a sulfur-containing aminoacid produced during metabolism of methionine. Since 1969 the relationship between altered homocysteine metabolism and both coronary and peripheral atherotrombosis is known; in recent years experimental evidences have shown that elevated plasma levels of homocysteine are associated with an increased risk of atherosclerosis and cardiovascular ischemic eve...

aminoacid sequences in common which are thought to bind hormones to specific sequences of DNA and modify the gene transcription (Beato, 1989). The complex formed by the binding of a steroid with a receptor is translocated into the cell nucleus where it binds to DNA and influences the expression of genes So the activation in depression of the HPA axis involves various steps in producing excess C...

Chemokines are a group of peptides of low molecular weight that induce the chemotaxis of different leukocyte subtypes [1]. The major function of chemokines is the induction of leukocyte migration to inflammation sites, but they also play a role in tumoral growth, in angiogenesis and in organ sclerosis [2]. At the present more than 50 chemokines have been described, which can be classified in 4 ...

Collagen and cyclodextrins are well known as triple helical protein and oligosaccharides. Cyclodextrins are the simplest organic compounds, which exhibit complex formation with other organic molecules and hence are excellent models of enzymes. This property of cyclodextrins to form inclusion complexes is being exploited in the present investigation. In fibrillogenesis, collagens with intact tel...

The genes encoding the heat shock proteins HSP10 and HSP16 of the salmon pathogen Piscirickettsia salmonis have been isolated and sequenced. The HSP10 coding sequence is located in an open reading frame of 291 base pairs encoding 96 aminoacids. The HSP16 coding region was isolated as a 471 base pair fragment encoding a protein of 156 aminoacids. The deduced aminoacid sequences of both proteins ...

In S. cerevisiae, the large oxi3/oli2 mitochondrial transcript contains the products of the oxi3, aap1 and oli2 genes and an unassigned reading frame, RF3. In the work presented here, we have completed the nucleotide sequence of RF3. We have shown that RF3 is composed of four fairly large ORFs which overlap within GC rich sequences. Furthermore, a shift of +1 base was found between each pair of...