The application of the mean force field in protein mutant stability prediction is explored. Based on protein main chain characteristics, including polar fraction, accessibility and dihedral angles, the mean force field was constructed to evaluate the compatibility between an amino acid residue and its environment, from which a position-dependent protein mutant profile was constructed. At each p...

An entire cycle of preclinical studies 4-[(3-ethoxy-3-oxopropanoyl)amino]benzoic acid (etmaben) exhibiting cardiotropic action was carried out. Its potential mechanism and pharmacological effects, general toxic effects in acute chronic experiments, specific types toxicity (allergenicity, immunotoxicity, reproductive toxicity, mutagenicity), pharmacokinetics were studied. The drug found to have ...

BACKGROUND We have previously performed a Genome Wide Association and linkage study that indicated a new disease triggering mechanism involving amino acid metabolism and nutrient sensing signaling pathways. OBJECTIVE The aim of this study was to investigate if plasma amino acid levels differed among children with celiac disease compared with disease controls. MATERIALS AND METHODS Fasting p...

It is important to be able to characterise foods and feedstuffs according to their available amino acid contents. This involves being able to determine amino acids chemically and the conduct of bioassays to determine amino acid digestibility and availability. The chemical analysis of amino acids is not straightforward and meticulousness is required to achieve consistent results. In particular a...

A growing demand for plant-based protein has seen a resurgence in the utilisation of pulses such as lentil and chickpea protein-rich substrate to produce food products traditionally based on animal or cereal protein. Additionally, offer metabolic benefits due bioactivity compounds including phenolic acids, simple complex carbohydrates more complete amino-acid profile which is not found grains. ...

The cDNA coding for Penicillium purpurogenum alpha-galactosidase (alphaGal) was cloned and sequenced. The deduced amino acid sequence of the alpha-Gal cDNA showed that the mature enzyme consisted of 419 amino acid residues with a molecular mass of 46,334 Da. The derived amino acid sequence of the enzyme showed similarity to eukaryotic alphaGals from plants, animals, yeasts, and filamentous fung...

Background Supplemental protein sources vary in composition in regards to size, structure, and amino acid composition of the varying individual proteins. Serum albumin concentrate (SA) is a new protein source extracted from edible-grade bovine plasma, which was recently made available on commercial scale. As compared to the low MW proteins in whey protein concentrate (WPC), SA consists of highe...

We recently characterized a transposon-induced NaCl-sensitive mutant of Staphylococcus aureus (U. Vijaranakul, M. J. Nadakavukaren, D. O. Bayles, B. J. Wilkinson, and R. K. Jayaswal, Appl. Environ. Microbiol. 63:1889-1897, 1997). To further characterize this mutant, we determined the nucleotide sequence at the insertion site of the transposon on the S. aureus chromosome. Nucleotide sequencing r...