In the course of an investigation of the chemical phosphorylation of ribonucleasel (cf. (l)), it became necessary to develop a chromatographic procedure for the fractionation of the phosphorylated protein. The method of Hirs et al. (2), employing the weak cation exchanger Amberlite XE-64, is an extremely valuable method for the chromatographic purification of pancreatic ribonuclease, but the us...

A new antifungal antibiotic, named ileumycin, was isolated from culture broth of streptomyces H 698-SY2, which was identified as S. lavendulae. The antibiotic was recovered from the culture filtrate by adsorption on Amberlite XAD-II and elution with aqueous methanol and was further purified by ion-exchange column chromatography on SE-cellulose and followed by partition chromatography on silica ...

by using special staining reagents on TLCshould result in interesting precursors for alkaloid synthesis. The screening was performed by centrifugation of the culture-broth from different Streptomyces strains, adsorption of the compounds in the culture filtrate on Amberlite XAD-16, elution with methanol and concentration. Detection of the amines after TLC separation of the products was done usin...

A method of determining histidine decarboxylase activity was established. In this method, 14C-histamine was separated from 14C-histidine by Amberlite CG-resin column in the ammonium form. Lecanoric acid was obtained by screening histidinedecarboxylase inhibitors produced by microorganisms. It is the first isolation of this compound from fungi. The inhibition by lecanoric acid was competitive wi...

A toxin, designated Fvrr has been isolated from the venom of the green mamba Dendroaspis angusticeps by ion exchange chromatography on Amberlite CG-50 and gel filtration on Sephadex G-50. . Toxin FvII consists of 61 amino acid residues, is devoid of phenylalanine and tryptophan, and is cross-linked by four intramolecular disulfide bridges. Immunochemically, toxin FvII is completely distinct fro...

A simple spectrophotometric flow injection (FI) procedure for the determination of Cr(III) using ethylenediaminetetraacetic acid (EDTA) was developed. An FI system with a column packed with Amberlite IR-120(H) was employed for sample pretreatment. This leads to the possibility of a single standard calibration. A linear calibration in a range of 10-27 microg Cr(III) was obtained with a detection...

Shackleton et al. (1), Osawa and Siaunwhite 2), and Beckloff et al. (3) have recently reported simple methods for assay of steroid conjugates in urine by use of columns of Amberlite XAD-2. With Osawa and Slaunwhite’s procedure urinary estrogen conjugates can be assayed with a lower limit of detection in the micromolar range but, as implied by the authors, the assay is useful only for analysis o...

No. Mei0279 Imaging the Distribution and Secondary Structure of Enzymes on Different Matrixes Using Infrared Microspectroscopy Ying Mei, Yong Chen, Richard Gross (Polytechnic Univ.) and Lisa Miller (NSLS) Beamline(s): U10B Introduction: Enzymes catalyze a plethora of reactions with high specificity, speed, and yield. However, in order to be useful as industrial biocatalysts, improvements in enz...