نتایج جستجو برای: alkaline protease
تعداد نتایج: 87344 فیلتر نتایج به سال:
Protease activity of actinomycetes isolated from various soil types of Georgia has been studied. Protease activity strains have been identified among mesophilic and extremophilic actinomycetes. Some strains of mesophilic, alkaliphilic and halophilic actinomycetes are characterized by alkaline protease activity. Their pH optimum for enzyme activity is 10-11. Strains of mesophilic actinomycetes: ...
This work verifies that amitraz and oxalic acid treatment affect honeybee cuticle proteolytic enzymes (CPE). Three bee groups were monitored: oxalic acid treatment, amitraz treatment, control. Electrophoresis of hydrophilic and hydrophobic CPE was performed. Protease and protease inhibitor activities (in vitro) and antifungal/antibacterial efficiencies (in vivo), were analyzed. Amitraz and oxal...
Alkaline protease producing microorganism Vibrio metschnikovii NG155, isolated from the soil samples of leather industry, having potential application in dehairing of animal skin was used in this study. To enhance the production of protease by this strain, various physical and nutritional factors were optimized using statistical methods like Plackett Burman and Response Surface Methodology. In ...
Animal fleshing, the major proteinaceous solid waste generated from leather industry, was used as substrate for the production of alkaline protease by Bacillus cereus 1173900 in solid-state fermentation (SSF). Maximum protease activity of 12,310 U/g was observed at 60 h in SSF crude extract. The extracted protease enzyme was purified by 53.64-fold through ammonium sulphate precipitation and chr...
An alkaline protease gene (Eap) was isolated for the first time from a marine fungus, Engyodontium album. Eap consists of an open reading frame of 1,161 bp encoding a prepropeptide consisting of 387 amino acids with a calculated molecular mass of 40.923 kDa. Homology comparison of the deduced amino acid sequence of Eap with other known proteins indicated that Eap encode an extracellular proteas...
Extra cellular alkaline protease producing species is an isolate from soil which was characterized and identified as Streptomyces pulvereceus MTCC 8374. Studies on submerged fermentation revealed that maximum level of enzyme production was during early stationary phase. Optimum pH, inoculum and temperature were 9.0, 3 percent and 33 C respectively. Among carbon sources 0.3 peccent starch gave a...
The nutritional medium requirement for alkaline protease production was optimized by newly isolated Bacillus amyloliquefaciens B7. The variables significantly influencing the protease activity were screened through one-factor-at-a-time experiments and Plackett–Burman design, by which fructose and yeast extract were identified as the most significant variables. The steepest ascent method was emp...
Proteases are very important industrial enzymes, which contribute about 60% of the total world enzyme market. Among the various proteases, bacterial proteases are the most significant, compared with animal and fungal proteases (Rahman et al., 1994) and among bacteria, Bacillus sp are specific producers of extra-cellular proteases (Coolbear et al., 1992; Banerjee et al., 1999). These enzymes hav...
Bacillus is remarkable for its extracellular enzyme production (Priest, 1977). When Bacillus cells exhaust the nutrients of the environment and enter into the stationary phase, neutral and alkaline proteases encoded by nprE and aprE respectively are produced and secreted to the outside environment (Chaloupka and Kreckova, 1966; Din et al., 1969). The mechanism of aprE expression has been extens...
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