105 Abstract: Origin of A-type alkali feldspar granites is currently the subject of a world-wide debate. Contrasting hypotheses have been proposed, which range from an entirely crustal origin to an almost complete mantle derivation. A-type alkali feldspar granites belong to either unimodal granite (rhyolite)-dominated association, or bimodal gabbro (basalt)-granite (rhyolite) suite. It is argue...

An automated method is described for determining alkaline phosphatase activity in serum, with use of sodium thymolphthalein monophosphate as the substrate. The system makes use of standard AutoAnalyzer components, has a simple flow diagram, and does not involve dialysis. The method has good precision, and the results correlate well with those from the manual method of which it is an adaptation.

Purified chicken intestinal alkaline phosphatase is active at pH 8 to 9, but becomes rapidly inactivated with change of pH to 6 or less. Also, a solution of the inactivated enzyme at pH 4.5 rapidly regains its activity at pH 8. In the range of pH 6 to 8 a solution of purified alkaline phosphatase consists of a mixture of active and inactive enzyme in equilibrium with each other. The rate of ina...

inor i8obutanol. Removal of the lipid is accompanied by simultaneous release of the enzyme into true solution. No other organic solvent tested was effective in releasing the bound phosphatase, indicating a unique action of butanol. I wish to thank Dr M. Dixon, F.R.S., for his encouraging interest and advice during this work. This work was carried out while holding a Travelling Scholarship award...

The increased alkaline phosphatase (EC 3.1.3.1) activity of reconstituted lyophilized control sera has recently been the focus of considerable interest because of its possible implications for quality-control procedures. We confirm that these reconstituted materials show a temperature-dependent increase in alkaline phosphatase activity, but could show no alteration in activity of fresh sera. Th...

The human alkaline phosphatases constitute a system of multiple molecular forms of enzymes in which heterogeneity is partly due to genetic factors and partly to posttranslational modifications. Recognition of the nature and occurrence of these multiple forms has made a significant contribution both to the understanding of changes in alkaline phosphatase values for serum in disease and to the us...

A perspective on serum alkaline phosphatase isoenzymes in liver disease is provided with a brief discussion of the location of the enzyme in liver and its presumed function. Mechanisms of entry of alkaline phosphatase into serum in liver disease are discussed. Characterization of high molecular weight alkaline phosphatase in obstructive jaundice is reviewed. The relationship between blood group...

phatase may reflect enzyme contributions from bone, liver, and intestine. We have investigated serum alkaline phosphatases in two siblings with hypophosphatasia. After administration of long-chain triglycerides, the major alkaline phosphatase component of their sera was shown to be of intestinal origin on the basis of inhibition by l-phenylalanine. Starch block electrophoresis suggested that th...

Reference ranges for amniotic fluid alkaline phosphatase, gamma-glutamyltransferase, and 5-nucleotidase are described from 13 to 40 weeks' gestation. Gamma-glutamyltransferase and 5-nucleotidase activities peak early in the second trimester and then decrease to low values. Alkaline phosphatase shows a similar pattern of activity from 13 to 29 weeks' gestation, but thereafter activity increases ...

Alkaline crude enzymes from the viscera of the Tunisian barbel (Barbus callensis) were extracted and characterized. Proteolytic crude extract from barbel viscera was active and stable in alkaline solution. The optimum pH and temperature were 11.0 and 55 °C, respectively, using casein as a substrate. The crude alkaline protease was extremely stable in the pH range of 5.0-12.0. Zymography activit...