the goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native iranian microorganisms. at the end of screening program, a bacillus subtilis bp-36 strain producing thermophilic alkaline protease was isolated from a hot spring in ardebil province. the target enzyme was purified using a one-step aqueous two-phase systems (atps) prot...

alkaline phosphatase (ortho phosphate, monoester hydrolase phosphoinositide e. c. 3.1.3.1) is a non-specific metalloproteinase enzyme that is located inside periplasmic space of bacteria. this enzyme is used to measure freshwater sediment in genetic engineering for cleaning water. isolation and molecular identification of the bacteria producing alkaline phosphatase and comparison of its product...

introduction: hydatid cyst disease is caused by the protoscolices of echinococcus granulosus. alkaline phosphatase (alp) enzyme is required for metabolism, physiology, immunology, and nutrients absorption in parasite. the aim of this study was to compare the level of alp activity (as a pathological biomarker) in hydatid cyst protoscolices (hcp) with that of sheep liver tissue and to determine t...

the activity of mouse endometrial alkaline phosphatase after hyperstimulation and progesterone injection at the implantation time alkaline phosphatase (alp) of endometrium may play a critical function in the development and implantation of embryo. the aim of this study was to determine the localization of endometrial alp activity after hyperstimulation and progesterone injection. thirty adult f...

background: the problem of bone defects during treatments of bone lesions, especially cranial trauma, and also during craniotomy operations indicates isolation of osteoblasts and culturing them for filling these bone effects. methods: bone tissue specimens were obtained from 6 patients’ carvaria undergoing craniotomy surgery operations in al-zahra teaching hospital and transferred to lab. these...

background: the present study was carried out on the human population of kashmir valley to evaluate the status of the biochemical parameters of infected population. materials and methods: blood samples were collected from 514 individuals, 298 (57.97%) males and 216 (42.02%) females, 187 (36.38%) were found sero-positive for human toxocariasis. results: alkaline phosphatase level was found highe...

an experiment was conducted to evaluate the extracted (es) and mechanical (ms) soybean meals two levels 25% and 35% in broiler chicken feeding. three hundred and fifty two one-day-old unsexed lohman chickens were placed in four treatments by 4 replicates and 22 chickens in each in a 2×2 factorial arrangement. feed intake was increased significantly in starter period by es in comparison with ms....

objective: osteoporosis or silent disease is a major bone disorder in elderly women in current century. estrogen has an important role in osteogenesis and prevention of bone fractures. hormone replacement therapy (hrt) is usually accompanied by such effects as breast and ovary cancers. thus, there is an increasing demand for replacement with plant phytoestrogens. this study is focused on determ...

in this study cellular phosphatase activity, referred to as enzyme developed color (edc) assay, was used for an in vitro assessment of cell proliferation and cytotoxicity. optimum conditions were established and found to be 10 x103 cells / well at 37°c and 60 min incubation (for developing color). under the same conditions a direct correlation between optical density (00) and cell number was ob...

background: pentachlorophenol (pcp) is one of the most fungicides and pesticides. acute and chronic poisoning from pcp may be occurred by dermal absorption, and respiration or ingestion. with respect to health and environmental effects of pcp, many methods were considered regarding its removal. microwave assisted other methods are environmental friendly, safety, and economical method, consequen...