1. Reaction of 2-(N-acetoxy)-acetamidofluorene with orthophosphate buffer at pH7 yielded a large quantity of water-soluble fluorene derivatives, which showed absorption peaks at 303, 290 and 280nm. Tris buffer under similar conditions gave negligible reaction. 2. Hydrolysis of polar material with acid or alkaline phosphatases liberated equimolar amounts of inorganic phosphate and an ether-extra...

A kind of degradable magnesium phosphate bone cement (MPBC) was fabricated by using the mixed powders of magnesium oxide (MgO), potassium dihydrogen phosphate (KH2PO4) and calcium dihydrogen phosphate (Ca(H2PO4)2.H2O). As MgKPO4, the main product of MgO and KH2PO4 was alkaline, the Ca(H2PO4)2.H2O was added to neutralize the alkali of the system. And the effects of Ca(H2PO4)2.H2O on the performa...

The production and repair of radiation-induced DNA damage were measured by filter elution in strains of mouse lymphoma L5178Y cells differing in their sensitivity to ionizing radiation. The induction of radiation-induced damage, as measured by filter elution at pH 12.1, 9.6, and 7.2, was similar in the resistant strain LY-R and the sensitive strain LY-S. The repair of single-strand breaks and a...

W7hen a resting skeletal muscle is treated with cold perchloric or other acid extracting agent, and the solution is analyzed with one of the standard methods for inorganic phosphate, values are obtained that differ for various kinds of muscle, but have been reported as about 5 pmoles per g for the sartorius muscle of the frog (I; see also Tables IV and V). For several reasons, we have felt the ...

Investigation of the electronic structure contending battery electrode materials is an essential step for developing a detailed mechanistic understanding charge–discharge properties. Herein, we use synchrotron soft X-ray absorption spectroscopy (XAS) in combination with complementary experiments and density functional theory calculations to map structure, band positioning, gap prototype vanadiu...

Heme is an essential protein cofactor used by nearly all forms of life to perform a wide range of tasks, from shuttling electrons to keep photosynthesis running to moving the oxygen we breathe from our lungs throughout our bodies (1). Most of the heme in humans is produced in erythroid cells, which can contain up to 1 billion heme molecules per cell (2). Because free heme that is not bound to p...

Cell surface hydrophobicity (CSH) of Candida species enhances virulence by promoting adhesion to host tissues. Biochemical analysis of yeast cell walls has demonstrated that the most significant differences between hydrophobic and hydrophilic yeasts are found in the acid-labile fraction of Candida albicans phosphomannoprotein, suggesting that this fraction is important in the regulation of the ...

1. Many of the glass containers commonly used in the laboratory, and most of the glass ampules employed in marketing sterile solutions for hypodermic or intravenous medication, yield sufficient alkali on autoclaving, to change the reaction of distilled water from pH 6.0 to pH 9.0. 2. This increase in alkalinity is sufficient to render biologically inert and partially to decompose aqueous soluti...

The broken efficiency of cell wall and the release amount of Pichia pastoris intracellular protein under different cell breaking conditions were investigated in this paper. The results showed that broken efficiency using hot alkali combined with high-pressure homogenizing method was higher than that of enzyme hydrolysis, hot alkali treatment and high-pressure homogenation, respectively. Suspend...