background: in the field of cellular therapy, the impact of confluence degree to harvest or differentiate bmmscs and the effect of cell-to-cell contact remains controversial. therefore, the effect of confluence on bmmscs properties was studied and confluence-associated osteogenic differentiation efficiency was identified. materials and methods: the impact of confluences at 20, 50, 70, 80 and 10...

Computer-assisted analysis of endothelial morphology provides useful indices of cell shape and size which appear to correlate to the monolayer's functional status. In this study morphometric data obtained by wide-field specular microscopy of in situ corneal endothelia are compared to data obtained by alizarin red S staining of excised corneas. Both human donor corneas and rabbit corneas were st...

The use of alizarin red S (ARS) marked tilapias could provide valuable fisheries management information to evaluate fish stocking events and may facilitate aquaculture management practices. As a new technique in fishes, the aim of this study was to compare and evaluate the chemical marks produced in tilapia juveniles by ARS through two treatments: 1) 12 hours of immersion and 2) immersion after...

Azo dyes are widely used in textile, printing, cosmetic, drug and food-processing industries. They are also used extensively in laboratories as either biological stains or pH indicators. The extent of such use is related to the degree of industrialization. Since intestinal cancer is more common in highly industrialized countries, a possible connection may exist between the increase in the numbe...

In guided bone regeneration (GBR), a barrier membrane is applied to provide space for promote cell adhesion, proliferation, and by inducing migration the surface of bone-deficient site. We prepared non-woven absorbable GBR comprised copolymer L-lactic acid glycolic (PLLGA) using dry spinning method. A thin dense comprising unsintered hydroxyapatite (uHA) poly-L-lactic (PLLA) was used comparison...

BACKGROUND The aim of this study is to identify which possessed the best stem-cell-like characteristics in 3 kinds of cell in human degenerative intervertebral disc: NPSCs (nucleus pulposus-derived stem cells), AFSCs (annulus fibrosus-derived stem cells), or CESCs (cartilage endplate-derived stem cells). METHODS We separated the disc samples obtained from 15 surgically treated patients with d...

This study examined the effects of BMP7 gene transfer on corneal wound healing and fibrosis inhibition in vivo using a rabbit model. Corneal haze in rabbits was produced with the excimer laser performing -9 diopters photorefractive keratectomy. BMP7 gene was introduced into rabbit keratocytes by polyethylimine-conjugated gold nanoparticles (PEI2-GNPs) transfection solution single 5-minute topic...