Immunochemical studies using polyclonal antisera prepared individually against highly purified cytosolic and chloroplast spinach leaf (Spinacia oleracea) fructose bisphosphate aldolases showed significant cross reaction between both forms of spinach aldolase and their heterologous antisera. The individual cross reactions were estimated to be approximately 50% in both cases under conditions of a...

We substituted neutral amino acids for some positively charged residues (R42, K107, K146, R148 and K229) that line the active site of aldolase A in an effort to determine binding sites for inositol 1, 4,5-trisphosphate. In addition, D33 (involved in carbon-carbon bond cleavage) was mutated. K229A and D33S aldolases showed almost no catalytic activity, but Ins(1,4,5)P(3) binding was similar to t...

Fructose diphosphate aldolase and glyceraldehyde 3-phosphate dehydrogenase have been crystallized from rabbit livers after single or sequential injections of [‘“Cland (3H]lysine. The specific radioactivity of the lysine in the dehydrogenase rises continuously and reaches a maximum during the 5th day after injection, whereas that of the aldolase declines exponentially from the middle of the 1st ...

Environmentally friendly hybrid-catalysis involving an aldolase and N-heterocyclic carbene gold catalyst for the one-pot preparation, with total atom economy, of various high added value monosaccharides from simple achiral reagents.

The essential and ubiquitous enzyme fructose bisphosphate aldolase (FBPA) has been a good target for controlling the various types of infections caused by pathogens and parasites. The parasitic infections of nematodes are the major concern of scientific community, leading to biochemical characterization of this enzyme. In this work we have developed a small dataset of all types of FBPA sequence...

4-Hydroxy-2-keto-pentanoic acid aldolase from Escherichia coli was identified as a class I aldolase. The enzyme was found to be highly selective for the acetaldehyde acceptor but would accept alpha-ketobutyric acid or phenylpyruvic acid in place of the pyruvic acid carbonyl donor.

Previously, we demonstrated that the vacuolar-type H(+)-ATPase (V-ATPase) a2-subunit functions as an endosomal pH sensor that interacts with the ADP-ribosylation factor (Arf) guanine nucleotide exchange factor, ARNO. In the present study, we showed that ARNO directly interacts not only with the a2-subunit but with all a-isoforms (a1-a4) of the V-ATPase, indicating a widespread regulatory intera...

It is generally accepted that the mechanism of enzyme activity includes a combination of enzyme and substrate. This concept forms the basis for the conventional kinetic analyses of enzymatic reactions (1). Direct evidence for the existence of enzyme-substrate combinations is as yet meager. The binding of pyridine nucleotide coenzymes as substrates to various dehydrogenases has been shown to res...

Case presentation: An 8-year-old girl, born from consanguineous parents, was admitted with a history of difficulty getting up the floor since second year life. Thereafter, she developed muscle pain, exercise intolerance (particularly walking long distances) and evident hyperlordosis. On neurological examination, there flaccid proximal-predominant tetraparesis. There no evidence sensory or cardi...