Interest in developmental interactions involving the notochord and perinotochordal sheath led to a comparative investigation of these structures in three mouse mutants. Alcian blue or periodic acid-Schiff staining of 9 1/2-13 days' gestational age embryos revealed a supernumerary notochordal-like mass of cells or a deflected notochord in association with duplication of the neural tube in mice o...

PURPOSE To study the glycoconjugates in the lacrimal sac epithelium of Japanese white rabbits with experimentally induced chronic dacryocystitis. METHODS Chronic dacryocystitis was induced by a subcutaneous injection of albumin followed by an injection of Staphylococcus aureus into the lacrimal sac. The histological appearance of the lacrimal sac was studied using the alcian blue-periodic aci...

BACKGROUND Systemic sclerosis or scleroderma is a rare collagen disease, characterized by insufficient angiogenesis. Few data are available about the morphologic and histochemical peculiarities of the skin in these patients with this condition. The purpose of the present work was to evaluate the histochemical aspects of sclerodermic skin, obtained through biopsy of the typical lesions from the ...

Mucins are produced by both benign and malignant gastric epithelium. In general, mucins can be classified into neutral and acidic mucins. The latter are of 2 major types, sulphated (sulphomucins) and carboxylated (sialomucins). A retrospective study was initiated at the Department of Pathology, University Hospital, Kuala Lumpur to histochemically study the mucin profiles of cases of intestinal ...

Histochemical quantification of goblet cells and measurements of the epithelial thickness were made in the trachea of male and female rats exposed to fresh whole tobacco smoke for 30 consecutive days. Exposure to tobacco smoke resulted in an increase in the total goblet cell count in both sexes, with the female rats differentially responding to a greater degree than the male rats. There was a s...

Objective. To find a convenient and efficient way to isolate MSCs from human menstrual blood and to investigate their biological characteristics, proliferative capacity, and secretion levels. Methods. MSCs were isolated from menstrual blood of 3 healthy women using adherence. Cell immunological phenotype was examined by flow cytometry; the adipogenic, osteogenic, and chondrogenic differentiatio...

The carbon and nitrogen content of transparent exopolymer particles (TEP) was determined and related to the concentration of TEP as quantified by a colorimetrical method. TEP were produced in the laboratory from dissolved precursors by laminar or turbulent shear. Dissolved precursors were obtained by 0.2 μm filtration from diatom cultures, with or without nutrient reduction, and from natural di...

Negative charges on red blood cell membranes were measured by the alcian blue binding test in 11 workers with high exposure to cadmium. Compared with 12 age matched control subjects, cadmium workers showed a significant decrease in red blood cell charge, which on average paralleled both the cadmium body burden and protein excretion. Animal data confirm these observations and also show that the ...

An upper bound on the magnitude of semicurcular canal cupula motion was experimentally determined in the isolated labyrinth of the skate, Raja erinacea. To visualize the cupula, a glass pipette was pushed through the ampullary wall, and local regions of the cupula were stained by slow infusion of small amounts of Alcian Blue dye. Caloric stimuli which produced large changes in single unit activ...

Patterns of cell division, cell death and chondrogenesis in cultured aggregates of normal and talpid 3 mutant chick limb mesenchyme cells SUMMARY Aggregates were prepared from dissociated mesenchyme cells obtained from normal and talpid 3 mutant chick limb buds at stage 26 and were maintained for 4 days in culture. They were shown by autoradiographic techniques to consist initially of populatio...