In this study, approaches were developed to examine the phenotypes of nonviable clinical varicella-zoster virus (VZV) strains with amino acid substitutions in the thymidine kinase (TK) (open reading frame 36 [ORF36]) and/or DNA polymerase (Pol) (ORF28) suspected to cause resistance to antivirals. Initially, recombinant TK proteins containing amino acid substitutions described as known or suspec...

Human herpesvirus-6 (HHV-6), human herpesvirus-7 (HHV-7), Epstein-Barr virus (EBV), and human cytomegalovirus (CMV) DNA were repeatedly assayed in peripheral blood leukocytes from 37 allogeneic bone marrow transplant (BMT) patients by polymerase chain reaction. Before BMT, HHV-6 DNA was detected in 8 (22%) patients. HHV-7, EBV, and CMV DNA were detected in 21 (57%), 10 (27%), and 1 (3%) patient...

The course of herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) and varicella-zoster virus (VZV) infections in squamous epithelial cells cultured in a three-dimensional organotypic raft culture was tested. In these raft cultures, normal human keratinocytes isolated from neonatal foreskins grown at the air-liquid interface stratified and differentiated, reproducing a fully differentiated ep...

STUDY OBJECTIVE To compare the effects of noninvasive assist-control ventilation (ACV) and pressure support ventilation (PSV) by nasal mask on respiratory physiologic parameters and comfort in acute hypercapnic respiratory failure (AHRF). DESIGN A prospective randomized study. SETTING A medical ICU. PATIENTS AND INTERVENTIONS Fifteen patients with COPD and AHRF were consecutively and rand...

HYPOTHESIS Donor management with airway pressure release ventilation (APRV) improves oxygenation and increases lung donation while maintaining equivalent graft survival. DESIGN Retrospective case series. SETTING Private, tertiary care, level I trauma center. PATIENTS Forty-five consecutive organ donors. INTERVENTIONS Management with assist/control ventilation (ACV) or APRV. MAIN OUTCO...

Resistance to aciclovir (ACV) among Herpes simplex virus (HSV) isolates is increasingly being reported in the literature particularly in immunocompromised patients. However, there is only limited data available from India despite widespread use of ACV in our hospital. A cross-sectional study was hence conducted to determine the aciclovir (ACV) susceptibility of HSV 1 and 2 isolates using a dye ...

9-([2-Hydroxy-1-(hydroxymethyl)ethoxy]methyl)guanine (BW B759U) is more potent and has a more prolonged inhibitory effect against Epstein-Barr virus (EBV) in vitro than does acyclovir (ACV). To assess the mechanism of this difference, we first compared the extent of phosphorylation of the two drugs in superinfected Raji cells. BW B759U is phosphorylated to levels 100-fold higher than is ACV. In...

PURPOSE The aim of this study was to compare an automated confrontation visual field testing (ACV) device with traditional finger-counting confrontation visual field testing (FCV). METHODS Forty-five eyes of 45 subjects with glaucoma, 5 eyes of 5 subjects with neurologic disease and 15 eyes of 15 normal subjects (age matched to the subjects with glaucoma by frequency) were tested on both ACV ...

OBJECTIVE This study aims to investigate the changes of anterior chamber morphology after laser peripheral iridotomy (LPI) in primary angle-closure (PAC) patients using Pentacam. METHODS 35 normal persons and 35 patients of PAC before and 1 week after neodymium: yttrium: aluminum garnet (Nd: YAG) LPI were evaluated with Pentacam. We measured the anterior chamber angle (ACA), central anterior ...

The majority of cases of acute encephalitis remain of unknown etiology despite extensive diagnostic evaluations [1, 2]. Herpes simplex virus (HSV) is the most common identified cause of acute sporadic encephalitis in the Western world, accounting for approximately 20% of total cases of identified cause [3]. Classic studies by the National Institute of Allergy and Infectious Diseases Collaborati...