نتایج جستجو برای: active site concentration

تعداد نتایج: 1100816  

Journal: :Biochemistry 2006
Anson M Nomura Alan B Marnett Nobuhisa Shimba Volker Dötsch Charles S Craik

Distinct mechanisms have evolved to regulate the function of proteolytic enzymes. Viral proteases in particular have developed novel regulatory mechanisms, presumably due to their comparatively rapid life cycles and responses to constant evolutionary pressure. Herpesviruses are a family of human pathogens that require a viral protease with a concentration-dependent zymogen activation involving ...

Journal: :The Biochemical journal 2000
J M Stevens R N Armstrong H W Dirr

We have shown previously that the solvent-induced equilibrium unfolding mechanism of class Sigma glutathione S-transferase (GST) is strongly affected by ionic strength [Stevens, Hornby, Armstrong and Dirr (1998) Biochemistry 37, 15534-15541]. The protein is dimeric and has a hydrophilic subunit interface. Here we show that ionic strength alone has significant effects on the conformation of the ...

Journal: :The Journal of biological chemistry 2010
Nicholas C Fitzkee James E Masse Yang Shen David R Davies Ad Bax

The human immunodeficiency virus type 1 (HIV-1) integrase (IN) is a critical enzyme involved in infection. It catalyzes two reactions to integrate the viral cDNA into the host genome, 3' processing and strand transfer, but the dynamic behavior of the active site during catalysis of these two processes remains poorly characterized. NMR spectroscopy can reveal important structural details about e...

Several nonpeptide small molecules were designed as potential inhibitors of HIV protease and their structures were constructed by computer-aided molecular modeling and docked iwo the active site of HIV protease. Models of the complexes of inhibitors and the HIV protease were refined using nonbonded and H-bonding terms. The refined energy of selected complexes showed that the designed inhib...

Journal: :Protein science : a publication of the Protein Society 2001
W B Ou W Luo Y D Park H M Zhou

Porcine kidney 18 kD peptidyl-prolyl cis-trans isomerase (PPIase) belongs to the cyclophilin family that is inhibited by the immunosuppressive drug cyclosporin A. The chaperone activity of PPIase was studied using inactive, active, and alkylated PPIase during rabbit muscle creatine kinase (CK) refolding. The results showed that low concentration inactive or active PPIase was able to improve the...

Harvester ants are known as one of the most renowned bio-disturbances in the arid and semi-arid ecosystems that affecte vegetation by collecting seeds and harvesting plants. It seems that physiognomy of plant association in steppe shrub land of Roodshoor, Saveh, Iran has been highly changed by harvester ants’ activities that caused to conduct this research. The study was carried out on active a...

Journal: :The Biochemical journal 1989
O Geiger H Görisch

The soluble form of the homogeneous quinoprotein glucose dehydrogenase from Acinetobacter calcoaceticus is reversibly inactivated at temperatures above 35 degrees C. An equilibrium is established between active and denatured enzyme, this depending on the protein concentration and the inactivation temperature used. Upon thermal inactivation the enzyme dissociates into the prosthetic group pyrrol...

2017
Hassan Beydoun

In this chapter we formulate an ice active surface site based stochastic model of heterogeneous freezing with the unique feature of invoking a continuum assumption on the ice nucleating activity (contact angle) of an aerosol particle’s surface, that requires no assumptions about the size or number of active sites. The result is a particle specific property g that defines a distribution of local...

Journal: :Journal of bacteriology 2011
Ashalla M Freeman Beth M Mole Ruth E Silversmith Robert B Bourret

Two-component regulatory systems, in which phosphorylation controls the activity of a response regulator protein, provide signal transduction in bacteria. For example, the phosphorylated CheY response regulator (CheYp) controls swimming behavior. In Escherichia coli, the chemotaxis phosphatase CheZ stimulates the dephosphorylation of CheYp. CheYp apparently binds first to the C terminus of CheZ...

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