L-Asparaginase has potential as an anti-cancer drug and for prevention of acrylamide formation in fried and baked foods. Production of the enzyme by Bacillus licheniformis (RAM-8) was optimized by process engineering using a statistical modeling approach and a maximum yield of 32.26 IU/ml was achieved. The L-asparaginase exhibited glutaminase activity of only 0.8 IU/ml and would therefore be le...

Recent findings of a potential human carcinogen, acrylamide, in foods have focused research on the possible mechanisms of formation. We present a mechanism for the formation of acrylamide from the reaction of the amino acid asparagine and a carbonyl-containing compound at typical cooking temperatures. The mechanism involves formation of a Schiff base followed by decarboxylation and elimination ...

This application note describes the use of a quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation procedure. The QuEChERS method employs an extraction and cleanup protocol for the determination of acrylamide with C3-acrylamide as the internal standard. The analyte and internal standard were separated on a reversed phase C-18 column (2.1 mm × 150 mm, 3 μm) by isocratic e...

A small epidemic of acrylamide poisoning amongst workers in the construction industry is described. The clinical features of this condition are reviewed and two cases are described in which residual neurological problems persisted for over 1 year. The literature on acrylamide poisoning is reviewed and it is recommended that this substance should only be handled in the open air or in well ventil...

Electrospun nanofibers present a new and rapidly growing research area due to their pronounced micro and nano characteristics associated with high surface area to volume ratio. Poly(styrene-co-acrylamide) and polystyrene polymers were synthesized by boiling temperature soap free emulsion polymerization in aqueous medium with potassium peroxosulphate as the initiator. The resulting polymers were...

OBJECTIVE To update the mortality experience of a cohort of 8508 workers with potential exposure to acrylamide at three plants in the United States from 1984-94. METHODS Analyses of standardised mortality ratios (SMR) with national and local rates and relative risk (RR) regression modelling were performed to assess site specific cancer risks by demographic and work history factors, and exposu...

BACKGROUND Acrylamide, a probable human carcinogen, was detected in various heat-treated carbohydrate-rich foods in 2002. The few epidemiologic studies done thus far have not shown a relationship with cancer. Our aim was to investigate the association between acrylamide intake and endometrial, ovarian, and breast cancer risk. METHODS The Netherlands Cohort Study on diet and cancer includes 62...

conclusions with regard to the potent antioxidant properties of silymarin, the neuroprotective effects of this natural compound suggested in the current study may in part be mediated through inhibition of oxidative stress. results exposure to acr reduced cell viability in pc12 cells. pre-treatment of cells with silymarin (100 µm) significantly reduced acr-induced toxicity. in addition, administ...

Dogs exposed to acrylamide develop peripheral neuropathy and megaoesophagus. The properties of slowly adapting oesophageal mechanoreceptors were studied in six control and five acrylamide affected greyhounds in which the oesophagus had been surgically isolated under anaesthesia and oesophageal pressure was controlled while monitoring impulses in oesophageal afferent fibres in the cervical vagus...

This application note describes the use of a quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation procedure. The QuEChERS method employs an extraction and cleanup protocol for the determination of acrylamide with C3-acrylamide as the internal standard. The analyte and internal standard were separated on a reversed phase C-18 column (2.1 mm × 150 mm, 3 μm) by isocratic e...