BACKGROUND Non-Newtonian fluids occur in many relevant flow and mixing scenarios at the lab and industrial scale. The addition of acid or basic solutions to a non-Newtonian fluid is not an infrequent operation, particularly in Biotechnology applications where the pH of Non-Newtonian culture broths is usually regulated using this strategy. METHODOLOGY AND FINDINGS We conducted mixing experimen...

Two simple, selective and sensitive spectrophotometric methods are described for the determination of 6-aminopenicillanic acid (6-APA). The methods are based on the reaction of 6-APA with either bromophenol blue (BPB) or bromothymol blue (BTB), to give orange-red and green species, respectively. The coloured products are quantified spectrophotometrically at 625 and 616nm for BPB and BTB, respec...

The severe acute respiratory syndrome caused by COVID-19 is now a global catastrophic event. Currently thereis no approved drug or vaccine for the disease. Methylene blue (MB, oxidized form, blue color) has been used inmany different areas of clinical medicine, ranging from malaria to orthopedics. Leucomethylene Blue (reduced formof MB, colorless) may be applied for the treatm...

This paper describes the primary structures of two opsins of short-wavelength-absorbing visual pigments deduced from the mRNA sequences in the retina of the Japanese yellow swallowtail butterfly Papilio xuthus. A phylogenetic analysis of the amino acid sequences indicates that one of these visual pigments is of the ultraviolet-absorbing type and that the other is of the blue-absorbing type. We ...

AIMS/BACKGROUND To evaluate the extent of oxygen radical damage in the cornea after excimer laser ablation. METHODS The 193 nm argon fluoride excimer laser was programmed for an average fluence of 150 mJ/cm2, with a firing rate of 5 Hz and an ablation zone diameter of 6 mm. Phototherapeutic keratectomy was performed to remove 30 microns of epithelium and 50 microns of stroma from the corneas ...

The contribution of DNA damage to the effects of 193-nm excimer laser radiation on mammalian cells in culture was studied in order to evaluate the mutagenic potential of this UV wavelength in vivo. Two approaches were taken: measurement of pyrimidine dimer-specific endonuclease-sensitive sites/megabase and comparison of the 193-nm radiation-induced cytotoxicity in normal versus DNA repair-defic...

In this study, we investigated the genetic basis of seed shattering and dormancy in Hokuriku 193 and bred an NIL improved these traits. Analysis of an F3 population from Hokuriku 193 × Koshihikari revealed a general correspondence between seed shattering and genotypes at the qSH1 locus, suggesting a strong influence of this locus on the seed shattering in Hokuriku 193. An F2 population from [ms...

The dyeing of woolen yarn with eggplant peel, a natural colorant, has been studied. The main coloring component in this natural colorant is anthocyanin, which is an interesting class of dyes in the blue and red area. Eight mordants including alum, copper sulfate, ferrous sulfate, potash alum, nickel chloride, zinc sulfate, tin chloride, and potassium dichromate were used for mordanting the ...

5.1 DNA Sequence Determination 168 5.1.1 Principles of DNA Sequencing 168 5.1.2 Automated Fluorescent DNA Sequencing 169 5.1.3 RNA Sequencing by Reverse Transcription 170 5.2 Gene Cloning 170 5.2.1 Classical Cloning 170 5.2.2 The Polymerase Chain Reaction 173 5.3 Enzymes Useful in Gene Manipulation 174 5.3.1 Restriction Endonucleases 174 5.3.2 Other Nucleases 175 5.3.3 Polynucleotide Kinase 176...