A fractionation of sulphur isotopes was found in all hetabolic processes investigated except those in which elemental sulphur was the starting substrate for growth of Thiobacillus concretivorus and Chromatiurn sp. and for reduction by Saccharomyces cerevisiae. Except for polythionates formed during sulphide oxidation by T. concretivorus or Chromatiurn sp., the products of metabolism were enrich...

A polar fatty acid has been observed as a component of an ornithine-containing lipid of Thiobacillus thiooxidans. A comparison of thin layer chromatographic mobilities of reference compounds to those of the natural acid and its derivatives suggested that the acid was a 2-hydroxy fatty acid. The presence of a cyclopropane function in the acid was indicated by W-labeling experiments and infrared ...

Trudinger, P. A. (Division of Plant Industry, Canberra, Australia). Effect of thiol-binding reagents on the metabolism of thiosulfate and tetrathionate by Thiobacillus neapolitanus. J. Bacteriol. 89:617-625. 1965.-Iodoacetamide, N-ethyl maleimide (NEM), p-chloromercuribenzoate (CMB), Mercurochrome, and HgCl(2) inhibited the oxidation of thiosulfate to sulfate by Thiobacillus neapolitanus; tetra...

Gas mixture of H2S and NH3 in this study has been the focus in the research area concerning gases generated from the animal husbandry and the anaerobic wastewater lagoons used for their treatment. A specific microflora (mixture of Thiobacillus thioparus CH11 for H2S and Nitrosomonas europaea for NH3) was immobilized with Ca-alginate and packed inside a glass column to decompose H2S and NH3. The...

This study demonstrates the efficacy of a direct 5S rRNA assay for the characterization of mixed microbial populations by using as an example the bacteria associated with acidic mining environments. The direct 5S rRNA assay described herein represents a nonselective, direct molecular method for monitoring and characterizing the predominant, metabolically active members of a microbial population...

An antiserum raised against whole cells of Thiobacillus ferrooxidans was allowed to react with a variety of acidophilic and nonacidophilic bacteria in an enzyme-linked immunosorbent assay and an indirect immunofluorescence assay. Both experiments demonstrated that the antiserum was specific at the species level. This preparation was used to evaluate the role of T. ferrooxidans in the microbial ...