in order to investigate the presence of barley mild mosaic virus, 425 samples of barley and wheat from razavi khorasan, northern khorasan, golestan, yazd and western azarbayjan were collected in spring 2011. plolyclonal antiserum was used in order to detect baymv by das-elisa. the highest level of baymv infection was found in azarbayjan gharbi and detect inrazavi khorasan, northern khorasan and...

A rapid seminested polymerase chain reaction (PCR) method for the specific, sensitive detection of virulent Shigella spp. in spiked environmental water samples was developed. A set of primers specific for the invasion plasmid antigen gene (ipaH) of virulent Shigella spp. and enteroinvasive Escherichia coli produced a 620-bp fragment that was used as template for the seminested primer pair delin...

Specific and sensitive amplification of major outer membrane protein (MOMP) gene (ompA) DNA sequences of Chlamydia species with various MOMP genotypes was achieved by a two-step polymerase chain reaction (PCR). Degenerate, inosine-containing oligonucleotide primers homologous to the 5' and 3' ends of the translated regions of all chlamydial MOMP genes were used in a PCR to amplify a DNA fragmen...

Human snake bitten poisoning is a serious threat in many tropical and subtropical countries such as Iran. The best acceptable treatment of envenomated humans is antivenoms; however they have a series of economic and medical problems and need more improvements. In this study a combinatorial human immunoglobulin gene library against some of Iranian snakes venoms was constructed. Total RNA prepare...

This article describes the first detection of Cytauxzoon felis, using molecular techniques, in a naturally infected domestic cat from Brazil, South America. Coinfection with ‘Candidatus Mycoplasma haemominutum’ was also found. The molecular identification of the piroplasmid species was performed by Polymerase Chain Reaction (PCR) and sequencing analysis. A 284 pb fragment of the gene encoding t...

kruppel-like factors (klfs) are a highly related zinc-finger family of transcription factors implicated inthe regulation of the eukaryotic cellular growth and differentiation of a diverse set of cells in mammal. usingrt-pcr technique, a 456 bp cdna fragment encoding n-terminus part of a klf2b was isolated from the skinmucosa of common carp (cyprinus carpio) using two degenerative oligonucleotid...

Objective(s): β-thalassemia is one of the most common genetic disorders in the world. As one of the promising treatment strategies, fetal hemoglobin (Hb F) can be induced. The present study was an attempt to reactivate the γ-globin gene by introducing a gene construct containing KLF1 binding sites to the K562 cell line. Materials and Methods: A plasmid containing a 192 bp sequence with two repe...