UNLABELLED We report here the results of studies on the in vitro receptor binding affinity, in vivo tumor uptake and biodistribution of two 99m-Tc-labeled peptides. METHODS Peptides P587 and P829 were synthesized by N-alpha-Fmoc peptide chemistry, purified by reversed-phase HPLC and characterized by fast-atom bombardment mass spectrometry. The peptides were labeled with 99mTc by ligand exchan...

Technetium-99m (99mTc) has been used in nuclear medicine and in biomedical research to label molecular and cellular structures employed as radiotracers. Here, we have evaluated, on a DNA repair proficient Escherichia coli strain, the 99mTc decay inactivation and the influence of the (i) pre-treatment with metal ion chelators or of the (ii) treatment with a free radical scavenger on the protecti...

This study was designed to evaluate the clinical usefulness of delayed exercise images in 99mTc-tetrofosmin (TF) myocardial SPECT in the diagnosis of vasospastic angina pectoris. We studied 30 patients with vasospastic angina, 10 of 30 patients (group A) had both effort and rest angina, 20 of 30 patients (group B) had rest angina. A 370 MBq of TF was intravenously injected at peak exercise, and...

Objectives. The purpose of this study was to assess the efficacy of attenuation-corrected (AC) technetium-99m (Tc)tetrofosmin single-photon emission computed tomography (SPECT) in detecting viable myocardium compared to Ffluorodeoxyglucose (FDG) positron emission tomography (PET). Background. The role of Tc-labeled perfusion tracers in the assessment of myocardial viability remains controversia...

OBJECTIVES We investigated the diagnostic accuracy of 99mTc-3,3-diphosphono-1,2-propanodicarboxylic acid (99mTc-DPD) scintigraphy for differentiation of monoclonal immunoglobulin light-chain (AL) and transthyretin (TTR)-related cardiac amyloidosis. BACKGROUND Differential diagnosis between TTR-related and AL amyloidosis is often complex and time-consuming. METHODS Patients under routine obs...

The present studies performed in experimental animals demonstrate that electrical direct current cardioversion can produce skeletal muscle damage and increased technetium-99m stannous pyrophosphate (99mTc-PYP) uptake; in experimental animals the electrically damaged skeletal muscle shows necrosis with extensive calcium deposition. In addition, the frequent administration of high energy cardiove...

UNLABELLED Electrocardiographically (ECG) gated myocardial SPECT with (99m)Tc-tetrofosmin has been used widely to assess left ventricular (LV) function. However, the accuracy of variables using ECG gated myocardial SPECT with beta-methyl-p-(123)I-iodophenylpentadecanoic acid (BMIPP) has not been well defined. METHODS Thirty-six patients (29 men, 7 women; mean age, 61.6 +/- 15.6 y) with ischem...

UNLABELLED Antibodies accumulate nonspecifically in infectious foci due to the locally increased vascular permeability. This study describes a method of infection imaging in which 99mTc-DTPA (diethylenetriaminepentaacetic acid) is trapped at the target by a previously administered anti-DTPA monoclonal antibody. DTIn1. METHODS Rats with Staphylococcus aureus-infected calf muscle were injected ...

Bone scintigraphy with 99mTc diphosphonates may exhibit extraosseous lesions in addition to metastatic lesions. Multiple factors can affect extraosseous 99mTc methylene diphosphonate (MDP) uptake. Similar uptake pattern of 99mTc MDP and fluorine-18 fluorodeoxyglucose (18F FDG) in hepatic metastasis was not already notified. In our case, initial tumor necrosis and subsequent intracellular calcif...