Formalin-inactivated Venezuelan equine encephalomyelitis vaccine was prepared from virus grown in rolling-bottle cultures of chicken embryo cells. Trinidad strain virus was propagated in these cultures with a maintenance medium consisting of serum-free medium 199 containing 0.25% human serum albumin (USP) and antibiotics. Manipulation of multiplicity of inoculum (0.06 to 0.00006) and maintenanc...

The integration of a newly learned spoken word form with existing knowledge in the mental lexicon is characterized by the word form's ability to compete with similar-sounding entries during auditory word recognition. Here we show that although the mere acquisition of a spoken form is swift, its engagement in lexical competition requires an incubation-like period that is crucially associated wit...

SUMMARY Radioautographs were prepared from ascites tumor cells removed at appropriate time points from tumor-bearing mice that had received bleomycin (BLM)-' 4C in advance. Radio autography indicated LM4 C was adsorbed to the surface of the cell membrane of tumor cells at 2 hr. After 4 hr, @ C was incorporatedinto the cellsand waslocatedmainlyon the nuclear membrane. There was a sharp increase ...

Rats, monkeys, and humans can alter the size of their spinal stretch reflex and its electrically induced analog, the H-reflex (HR), when exposed to an operant conditioning paradigm. Because this conditioning induces plasticity in the spinal cord, it offers a unique opportunity to identify the neuronal sites and mechanisms that underlie a well-defined change in a simple behavior. To facilitate t...

The relationship between inhibition of polyamine biosyn thesis and interference with mitochondria! structure and func tion by the antitumor agent, methylglyoxal-bis(guanylhydrazone) (MGBG), was examined by studying the temporal sequence of events relevant to these two drug actions. In ascites L1210 cells treated in vivo with a single dose of MGBG (75 mg/kg), significant inhibition of pyruvate u...

Plasma cells obtained from the peripheral blood of a patient with multiple myeloma was incubated in serum and Krebs-Ringer bicarbonate buffer with (14)C-labeled glucose, acetate, and propionate. Glucose utilization by these cells amounted to 0.5 mumole per hr per 10(8) cells and was mainly via the Embden-Meyerhof pathway, and only 6% or less traversed the hexose monophosphate shunt. The presenc...

To the Editor: The recent article by Dhillon et al. (1) is of great interest to those working with curcumin. As the investigators suggest, the usefulness of curcumin could be minimized because of its poor oral bioavailability (2). Data from this study show that only 22 to 41 ng/mL were detectable in plasma even when 8 g curcumin/day was given orally (1). Curcumin levels in the microgram range h...

Abstract Introduction Traditionally, positive airway pressure (PAP) therapy is initiated in a face-to-face visit. This can be barrier to accessing care for rural Veterans. The COVID-19 pandemic led the implementation of telehealth PAP set-ups at two large VA sleep centers providing an opportunity assess effectiveness versus initiation Methods We performed retrospective cohort study Veterans (de...

BACKGROUND Interferon-based therapy (IBT) has been the standard of care for hepatitis C virus (HCV) infection. However, conflicting results exist regarding the effects of IBT on risk of developing hepatocellular carcinoma (HCC) and cirrhosis-associated complications, and most included highly selected patients. METHODS This 8-year cohort study was based on the Longitudinal Health Insurance Dat...

The Jarisch-Herxheimer Reaction (J-HR) is a clinical syndrome occurring soon after the first adequate dose of an antimicrobial drug to treat infectious diseases such as Lyme disease, syphilis, and relapsing fever. Previous attempts to identify factors mediating this reaction, that may cause death, have been unsuccessful. We conducted a prospective trial in Addis Ababa, Ethiopia on 17 patients t...