Asp(443) and Glu(478) are essential active site residues in the RNase H domain of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT). We have investigated the effects of substituting Asn for Asp(443) or Gln for Glu(478) on the fidelity of DNA-dependent DNA synthesis of phylogenetically diverse HIV-1 RTs. In M13mp2 lacZα-based forward mutation assays, HIV-1 group M (BH10) and...

Systematic, well-designed research provides the most effective approach to the solution of many problems facing highway administrators and engineers. Often, highway problems are of local interest and can best be studied by highway departments individually or in cooperation with their state universities and others. However, the accelerating growth of highway transportation develops increasingly ...

The emergence of multidrug-resistant enterococci (MDRE) and particularly vancomycin-resistant enterococci (VRE) is considered a serious health problem worldwide, causing the need for new antimicrobials. The aim of this study was to discover and characterize bacteriocin against clinical isolates of MDRE and VRE. Over 10,000 bacterial isolates from water, environment and clinical samples were scr...

The selected strains of microscopic fungi, Haematonectria haematococca (BwIII43, K37) and Trichoderma harzianum (BsIII33), decolorized the following monoathraquinone dyes with different efficiency: 0.03 % Alizarin Blue Black B, 0.01 % Carminic Acid, 0.01 % Poly R-478, and 0.2 % post-industrial lignin. The most effective was the removal of 0.03 % Alizarin Blue Black B (50-60 %) and 0.01 % Carmin...

The dry sliding friction and wear behaviors of TC11 titanium alloy subjected to the ultrasonic impacting and rolling process (UIRP) were studied in the present work. The microstructure of the deformation layer and the morphology of the worn surfaces were observed. The results clearly show that the wear performance of TC11 alloy after UIRP is better than that of TC11 alloy before UIRP under the ...

OBJECTIVE To investigate whether there is an association between chronic peripheral neuropathy and coeliac disease. METHODS The cause of chronic peripheral neuropathy was first investigated in a group of 478 patients. Published reports were then examined systematically for an association between chronic peripheral neuropathy and coeliac disease. Cases were divided into two groups: group A, po...

Within our activities on accelerator-based boron neutron capture therapy (BNCT) carried out at the Tandar Laboratory (Comisión Nacional de Energı́a Atómica, Argentina) we present here the study and design of a tomographic imaging system for the measurement of the spatial distribution of the absorbed dose during a BNCT session. The 10B(n,α)7Li boron neutron capture reaction produces a 478 keV gam...

Parvovirus B19 is a small DNA virus. Infection with parvovirus B19 during pregnancy may cause serious complications in the fetus, including hydrops fetalis and fetal death. The purpose of the present study is to clarify the clinical manifestations and outcomes of parvovirus B19 infection during pregnancy. This prospective study enrolled 478 women with suspected B19 infections during pregnancy b...