Whole cell recordings were performed on acutely dissociated neurons from the horizontal limb of the diagonal band of Broca (hDBB) from rats to elucidate the ionic mechanisms of action of neurotensin. Neurotensin caused a decrease in whole cell voltage-activated outward currents and failed to elicit a response when Ca2+ influx was blocked by changing the external solution to the one containing 0...

Recently, extensive behavioral research has been conducted on the benztropine (BZT) analogs with the goal of developing successful therapeutics for cocaine abuse. The present study was conducted to characterize the contribution of dispositional factors in mediating the behavioral differences among the chloro BZT analogs and to identify cytochrome P450 enzymes involved in their metabolism. Bidir...

The effect of the chemotatic peptide, N-formylmethionylleucylphenylalanine (FMLP), on actin conformation in human neutrophils (PMN) was studied by flow cytometry using fluorescent 7-nitrobenz-2-oxa-1,3-diazole (NBD)-phallacidin to quantitate cellular F-actin content. Uptake of NBD-phallacidin by fixed PMN was saturable and inhibited by fluid phase F-actin but not G-actin. Stimulation of PMN by ...

A direct injection, liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed for the analysis of the chloro-s-triazine herbicides and their degradates in finished drinking water. The target compounds in the method were selected based on their inclusion in a common mechanism group (CMG) because of their ability to induce a similar toxic effect through a common mechanis...

Mossbauer spectra of a polycrystalline form of oxidized chloro-S, 10, 15,20-tetra(mesityl)porphyrinatoiron(lIl) [Fe(TMP)Cl], compound A, were recorded over a range of temperatures (4,2-195 K) and magnetic fields (0-6 T), These spectra of compound A exhibit magnetic features which are markedly different from those of the analogous protein complexes, horse radish peroxidase compound I (HRP-I) and...

Background: many diseases are provoked by sunlight directly or indirectly. Skin damage occurs due to prolonged and high doses of UV on prematurely age the skin. These changes are predominantly caused by medium wavelengths and can occur in anyone with sufficiently high levels of UV exposure. Abnormal reactions to UV, however, are predominantly triggered by UV-A radiation and do not affect everyo...