Extensive effort has been made to develop a three-dimensional (3D) system for the culture of pluripotent stem cells in human and model animals, which yields lots of benefits for monitoring cell-to-cell or cell-to-environment interaction and for suggesting alternative materials for clinical cases. Initial study using animal model moved toward supporting embryonic stem cells (ESCs) self-renewal i...

to efficiently expand cells and develop robust cell-based models for in vitro drug screening, in vivo-like cell culture conditions — such as dynamic perfusion and 3d growth — are required. here, we show results on a study in which we cultured CompoZr® Zinc finger nuclease-modified U2oS osteosarcoma cells under 3d perfusion cell culture conditions. CompoZr Zinc finger nuclease (Zfn) technology w...

Evidence for the functional superiority of cells cultured as 3D aggregates or on 3D scaffolds over conventional 2D monolayer cultures has created interest in material and cell based methods that influence the formation and structure of multicellular aggregates in vitro. We have created a co-culture of primary rat hepatocytes and hepatic stellate cells on a poly(DL-lactic acid) surface, a poor s...

Three-dimensional (3D) cultures are increasing in use because of their ability to represent in vivo human physiology when compared to monolayer two-dimensional (2D) cultures. When grown in 3D using scaffold-free agarose hydrogels, MCF-7 human breast cancer cells self-organize to form directionally-oriented microtissues that contain a luminal space, reminiscent of the in vivo structure of the ma...

There is a need for functional in vitro 3D human intestine systems that can bridge the gap between conventional cell culture studies and human trials. The successful engineering in vitro of human intestinal tissues relies on the use of the appropriate cell sources, biomimetic scaffolds, and 3D culture conditions to support vital organ functions. We previously established a compartmentalized sca...

PURPOSE The major problem in producing artificial livers is that primary hepatocytes cannot be cultured for many days. Recently, 3-dimensional (3D) printing technology draws attention and this technology regarded as a useful tool for current cell biology. By using the 3D bio-printing, these problems can be resolved. METHODS To generate 3D bio-printed structures (25 mm × 25 mm), cells-alginate...

Dopaminergic differentiation of embryonic stem cells (ESCs) gains more and more attention worldwide owing to its potential use for neurorestorative therapy for the treatment of Parkinson's disease. The conventional 2D cell culture on petri dishes with various animal derived substrata such as collagen gels, laminin, and Matrigel is widely used to induce dopaminergic differentiation and it may li...

The cancer stem cell (CSC) population displays self-renewal capabilities, resistance to conventional therapies, and a tendency to post-treatment recurrence. Increasing knowledge about CSCs' phenotype and functions is needed to investigate new therapeutic strategies against the CSC population. Here, poly(ε-caprolactone) (PCL), a biocompatible polymer free of toxic dye, has been used to fabricate...