Insufficient glycogen content in bovine muscle at slaughter produces meat with high final pH (> 5.8) which is undesirable. The objective of this study was to determine through biopsies the live changes in muscle glycogen concentration (MGC) of housed steers fed on hay or hay plus energy concentrate for 30 d and then determine the effect of food deprivation (fasting) for 24 h on the same variabl...

This work presents new climate and emissions scenarios to investigate changes on future meteorology air quality in the U.S. Here, we employ a dynamically downscaled Weather Research Forecasting model coupled with chemistry (WRF/Chem) simulations that use two Intergovernmental Panel Climate Change (i.e., A1B B2) integrated explicitly projected from novel Technology Driver Model (TDM). The 2046–2...

Skeletal muscle responds to exercise-induced damage by orchestrating an adaptive process that protects the muscle from damage by subsequent bouts of exercise, a phenomenon called the repeated bout effect (RBE). The mechanisms underlying the RBE are not understood. We hypothesized that an attenuated inflammation response following a repeated bout of lengthening contractions (LC) would be coincid...

We measured the cleavage of angiotensin I (Ang I) metabolites by angiotensin I-converting enzyme (ACE) in cultured cells and examined how they augment actions of bradykinin B2 receptor agonists. Monolayers of Chinese hamster ovary cells transfected to stably express human ACE and bradykinin B2 receptors coupled to green fluorescent protein (B2GFP) or to express only coupled B2GFP receptors. We ...

The nrdB gene of Escherichia coli, coding for the B2 protein of ribonucleotide reductase, has been cloned in a runaway-replication vector. The runaway derivative pBEU17 carries the promoter-proximal portion of the E. coli alanyl-tRNA synthetase gene and proved useful for expressing cloned genes lacking their native transcription initiation signals. The alaS promoter is located approximately 500...