Lactate dehydrogenase (LDH) is commonly used in human cancer patients for prognostic purposes. Aim of this study was to determine the magnitude of serum LDH elevation in dogs with cancer compared with healthy dogs and dogs with non-neoplastic disease, and to verify whether it may support the diagnosis of specific malignancies. About 128 healthy dogs, 211 diseased dogs and 188 cancer dogs were e...

Fluorescence resonance energy transfer (FRET) with fluorescent proteins is a powerful method for detection of protein-protein interactions, enzyme activities and small molecules in the intracellular milieu. Aided by a new violet-excitable yellow-fluorescing variant of Aequorea victoria GFP, we developed dual FRET–based caspase-3 biosensors. Owing to their distinct excitation profiles, each FRET...

Four new lactone compounds, named feigrisolides A to D (1 to 4), have been isolated from Streptomyces griseus. The chemical structures were determined by detail analysis of their spectroscopic data and chemical transformations. Structurally, the feigrisolides A (1) and B (2) are hepta-lactones, feigrisolide C (3) and D (4) are 16-membered macrodiolides. Biological studies showed that feigrisoli...

The objective of this study was to investigate the different dietary ratios of n-6 to n-3 (n-6/n-3) fatty acid (FA) on performance and n-6/n-3 FA in muscles of broiler chickens. A total of 300 one-day-old Cobb chicks were randomly assigned to 3 treatments of 10 replicates in each (10 birds/replicate). Birds were fed on a corn-soybean meal-based diet containing 1% oil during starter (day 1 to 21...

A series of 3-alkyl(aryl)-4-[3-(2-metylbenzoxy)-4-methoxybenzylideneamino]-4,5-dihydro-1H-1,2,4-triazol-5-ones (1-9) were titrated potentiometrically with tetrabutylammonium hydroxide in four non-aqueous solvents such as isopropyl alcohol, tert-butyl acetonitrile and N,N-dimethylformamide (DMF), graphs drawn for all cases. The half-neutralization potential values the corresponding pKa determine...

Antigen-free media suitable for cultivating anaerobic bacteria from the human gingival crevice were devised, based upon supplementing diffusates from standard media ingredients. Growth in these is at least as good as in customary media. Formulations suitable for the major species encountered are described and compared. 概要:口 腔内嫌気性培養用に考案 した透析培地について,真 下 彰*,S・A・ エ リソン*.口 腔内諸 疾患 の免疫学的研究 を,と くに 口内細菌 ...

This work combines a theoretical study of electrical interactions between two excitable heart cells, using a variable coupling resistance, with experimental studies on isolated rabbit ventricular cells coupled with a variable coupling resistance to a passive resistance and capacitance circuit. The theoretical results show that the response of an isolated cell to an increased frequency of stimul...

one of the important bottle-necks in production of microalgae based biodiesel is thelack of an efficient method for harvesting of microalgae from the culture medium. in thisstudy, the electroflocculation method was developed for harvesting of microalgaedunaliella cells from culture medium. the effect of several parameters such as thecurrent density, electrical conductivity of culture medium, ti...

The effect of abscisic acid (ABA) and excess benzyladenine (BA) on the formation of shoot from tea (Camellia sinensis L. assamica × sinensis) leaf was investigated in this research. Callus was formed and grew well when explants were cultured on LS basal medium supplemented with (in mg/L) thiamine-HCl, 1.25; pyridoxine-HCl, 0.625; nicotinic acid, 0.625; indole-3-acetic acid (IAA), 30; naphthalen...

Octanoic and decanoic acid, the major constituents of the Medium Chain Triglyceride (MCT) Emulsion diet, have been detected in appreciable quantities in the peripheral blood of children with intractable seizures treated with the MCT diet. Serum concentrations of these acids as well as beta hydroxybutyrate and acetoacetate rose as the diet was introduced and on full diet showed pronounced diurna...