Hyaluronan (HA) hydrolysis catalysed by hyaluronidase (HAase) is strongly inhibited when performed at low HAase over HA concentration ratio and under low ionic strength conditions. The reason is the ability of long HA chains to form electrostatic and non-catalytic complexes with HAase. For a given HA concentration, low HAase concentrations lead to very low hydrolysis rates because all the HAase...

This study evaluated four fluorescent-protein conjugates to monitor microcirculatory variables using the murine cremaster muscle and determined acute and long-term responses to repeated administration of FITC-BSA [conjugated at the University of Sheffield (UoS)] within a dorsal microcirculatory chamber (DMC) in rats. For analysis of the cremaster muscle, male C3H/HeN mice were anaesthetized, th...

The characteristics of the interaction between reserpine and bovine serum albumin (BSA) were studied by fluorescence, UV-vis absorption and Fourier transform infrared (FT-IR) spectroscopy. Spectroscopic analysis revealed that fluorescence quenching of BSA by reserpine was through a static quenching procedure. The binding constant K(A) of reserpine with BSA at 293, 301 and 309 K was 1.63, 1.78 a...

The effect of gender and degree of obesity on the size indicators V, used to normalize urea clearance (Kt/Vur), and body surface area (BSA), used to normalize creatinine clearance (Ccr), in peritoneal dialysis was studied by: (1) mathematical comparison of the formulae used to estimate V (Watson and Hume) with the Dubois formula used to estimate BSA in peritoneal dialysis; and (2) comparison of...

In this paper, the quartz crystal microbalance with dissipation monitoring (QCM-D) was used to investigate hydrophobicity and binding strength (KD) for 10 different drugs interacting human serum albumin (HSA). Quantitative structure activity relationship (QSAR) analysis determine between drug (ClogP) HSA log(1/KD). The results are compared prior knowledge on bovine (BSA) binding. We demonstrate...

BACKGROUND Gold nanoclusters (AuNCs) are typically composed of several to tens of gold atoms which are stabilized with biomacromolecules such as bovine serum albumin (BSA). Au NCs fluoresces in the visible to near infrared region, in a size-dependent manner. AuNCs solutions have potential as fluorophore in a wide range of biomedical applications such as biodetection, biosensing and bioimaging i...

BSA solutions of 10 %, 1 % and 0.1 % were prepared by addition of lyophilized BSA powder (Sigma Aldrich, St Louis, MO, USA) into deionized water before being filtered through a glass fiber filter (Whatman GF/F) under vacuum in order to remove particles that can clog the system. Tween 80 was then added to a obtain 0.05 % [V/V] solution. The BSA solutions were then refrigerated before use. All so...

This study describes the fabrication of an aflatoxin (AFB1) electrochemical immunosensor by immobilization anti-AFB1 antibody-bovine serum albumin (BSA) conjugate on a layer L-cysteine (L-Cys) used to link antibody palladium nanoparticle-boron nitride (PdNP-BN)-modified carbon felt (CF) electrode. Each step was characterized using cyclic voltammetry (CV) and impedance spectroscopy (EIS). These ...