نتایج جستجو برای: 2neu gene quantification
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Alternative splicing generates multiple transcript and protein isoforms from the same gene and thus is important in gene expression regulation. To date, RNA-sequencing (RNA-seq) is the standard method for quantifying changes in alternative splicing on a genome-wide scale. Understanding the current limitations of RNA-seq is crucial for reliable analysis and the lack of high quality, comprehensiv...
Objective: To collect high quality, representative tissue material from tumors and manage its distribution to different laboratories. Design: Prospective controlled study. Animals: Thirty-six dogs with mast cell tumors. Procedures: The samples were submitted for the following analyses: stereology; histopathology; cell culture; breakdown for cytogenetic analysis of chromosomes (based on the Boxe...
The expression gene pattern reflects, in part, mechanisms involved in adaptation to environmental conditions. Thus, we established and validated a method that enables relative transcript quantification in different conditions in the lactic acid bacteria Oenococcus oeni, notably in a technological medium. First, we determined an internal control in our conditions by reverse transcription quantit...
A new method is proposed for improving microarray spot segmentation for gene quantification. The method introduces a novel combination of three image processing stages, applied locally to each spot image: i/ Fuzzy C-Means unsupervised clustering, for automatic spot background noise estimation, ii/ power spectrum deconvolution filter design, employing background noise information, for spot image...
It is currently unknown if the use of a real-time polymerase chain reaction (PCR) adds value to the diagnosis and follow-up prognosis of patients affected by leishmaniasis. We performed a study using a real-time PCR directed against the alpha-polymerase gene and a semiquantitative PCR that target the SSU ribosomal RNA (rRNA) gene as control for the diagnosis and quantification of parasites in p...
The notation and terminology used here are introduced in the following papers: [13], [14], [3], [9], [2], [7], [1], [4], [5], [10], [6], [12], [11], and [15]. The following proposition is true (1) For all real numbers x, y such that 0 ≤ x < y there exists a real number s0 such that 0 < s0 and x < y 1+s0 < y. The scheme RecExD3 deals with a non empty set A, an element B of A, an element C of A, ...
Linguistic quantifiers are quantifiers defined by linguistic expressions like “around 5” or “most of,” and many types of linguistic quantifiers can be found in the literature (Diaz-Hermida, Bugarin, & Barro, 2003; Glockner, 1997, 2004a, 2004b; Losada, Díaz-Hermida, & Bugarín, 2006) (as semifuzzy quantifiers which allow modeling expressions like “there are twice as many men as women”). We limit ...
Sugarcane (Saccharum spp. hybrids) is a world-wide cash crop for sugar and biofuel in tropical and subtropical regions and suffers serious losses in cane yield and sugar content under salinity and drought stresses. Although real-time quantitative PCR has a numerous advantage in the expression quantification of stress-related genes for the elaboration of the corresponding molecular mechanism in ...
Next-generation sequencing technologies offer new approaches for global measurements of gene expression but are mostly limited to organisms for which a high-quality assembled reference genome sequence is available. We present a method for gene expression profiling called EDGE, or EcoP15I-tagged Digital Gene Expression, based on ultra-high-throughput sequencing of 27-bp cDNA fragments that uniqu...
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