The performance of two-dimensional electrophoresis in conventional gels in Cartesian coordinates (2-DE) vs. polar coordinates (2-PE) is here evaluated. Although 2-DE is performed in much longer Immobiline gels in the first dimension (17 cm) vs. barely 7-cm in 2-PE, an equivalent resolving power is found. Moreover, due to the possibility of running up to seven Immobiline strips in the radial gel...

Trypanosoma cruzi trypomastigotes excrete-secrete a complex mixture of antigenic molecules. This antigenic mixture denominated trypomastigote excreted-secreted antigens contains a 150-160 kDa band that shows excellent performance in Chagas’ disease diagnosis by immunoblotting. The present study partially characterized by two-dimensional gel electrophoresis the immunoreactivity against the 150-1...

Recombinant monoclonal antibodies (rmAbs) are medicinal products obtained by rDNA technology. Consequently, like other biopharmaceuticals, they require the extensive and rigorous characterization of the quality attributes, such as identity, structural integrity, purity and stability. The aim of this work was to study the suitability of gel electrophoresis for the assessment of charge heterogene...

Immunological sperm sexing is one of the desirable choices to separate X and Y sperm. The basic concept of immunological technique is based on the different proteins on the surface of X and Y sperms. To investigate the sperm surface proteins, proteomic investigation using 2 dimensional gel (2D-gel) electrophoresis technique has been applied. The initial step, protein extraction is very importan...

Drought compromises edible vegetable production worldwide, including common bean (Phaseolus vulgaris L.) an economically important crop that is highly dependent on optimum rainfall or abundant irrigation. In the present study, phenotypic data of 26 Bulgarian mutant lines and cultivars subjected to drought stress has been summarized, reaction was evaluated by chlorophyll fluorescence proteomics ...

the domesticated silkworm, bombyx mori linn., a lepidopteran molecular model and an important economic insect that are emerging as an ideal molecular genetic resource for solving a broad range of biological problems. the silkworm, b. mori produces massive amount of silk proteins during the final stage of larval development. these proteins are stored in the middle silk gland and they are dischar...

background: failure in the treatment of burn patients infected with pseudomonas aeruginosa could happen as a result of the acquisition of antibiotic resistance, including carbapenems. objectives: the aim of the present study was to investigate the phenotypic and genotypic characteristics of the pseudomonas aeruginosa strains, isolated from burn patients. patients and methods: during a 12 month ...

morphological and electrophoretic analysis of the crystalline inclusions (parasporal crystals) of sporulated cultures of b. thuringiensis ssp. kumamotoensis dsm 6070 (bt 6070) was conducted via phase contrast and scanning electron microscopy and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. the activity of the spore-crystal suspensions against house fly (musca domestica) larvae wa...

stress is a threatening factor that all living organisms encounter throughout life. depending on the type of stress, there are several mechanisms for keeping body homeostasis to minimize stress effects. brain is an organ which shows high sensitivity to stress conditions. although many studies have shown induced-stress effects on rat embryos, little is known about the mechanisms involved in copi...