Dynamin 2 is a large GTPase notably involved in clathrin-mediated endocytosis, cell migration and cytokinesis in mitosis. Our previous study identified that Dynamin 2 regulated polar body extrusion in mammalian oocytes, but its roles in early embryo development, remain elusive. Here, we report the critical roles of Dynamin 2 in mouse early embryo development. Dynamin 2 accumulated at the periph...

The objectives of this s tudy were to test the effects of four levels of bovine serum albumin (BSA) on the development of ovine embryos cultured in Brinster's and Whitten's media and to examine the extent of in vitro development of ovine embryos cultured from various cell stages. Two hundred and forty-three oneto 16-celi ovine embryos were cultured in Brinster's medium (BMOC-2 plus .1% w/v dext...

The relationship between the level of cell confluence near the plateau phase of growth and blastocyst yield following somatic cell cloning is not well understood. We examined the effect of distinct cell culture confluence levels on in vitro development of cloned bovine embryos. In vitro-matured bovine oocytes were manually bisected and selected by DNA staining. One or two enucleated hemi-cytopl...

The correlation between cell-to-cell communication through gap junctions at the 32-cell stage and the subsequent patterning of the embryonic axis has been examined in Xenopus laevis embryos. Disturbances of embryonic axis formation were generated by exposure to u.v. irradiation at the vegetal pole before 0-6 in the cell cycle, which generates embryos with dorsal axial deficiencies. Alternativel...

Porcine embryos were flushed from mated donors and examined for cleavage stage. One- and two-cell embryos were randomly allotted to one of the five following in vitro treatments: M199 with Earle's salts, a modified Tyrode's medium (TL), TL supplemented with 10 mM N-2-hydroxyethyl-piperazine-N'-2-ethanesulfonic acid (HEPES) (TLH), TLH supplemented with 5.5 mM glucose (TLHG), or TLH supplemented ...