Random amplified polymorphic DNA (RAPD) analysis was used to evaluate genetic diversity among 13 soil Penicillium strains originating from widely dispersed areas. Twenty one of the 34 synthetic random primers were found to identify polymorphism in amplification products. The results show a high level of diversity of RAPD markers among the strains. All the strains could be identified by their ch...

Andrographis paniculata Nees. (AP) is a self-pollinated medicinal herb with a wide range of pharmaceutical properties, facing a low diversity in Malaysia. Cross-pollination of AP accessions leads to considerable rates of heterosis in the agro-morphological characteristics and anticancer phytochemicals of this eminent medicinal herb. However, the poor crossability of the plant at the interpopula...

First line in abstract should be Date palm is an important fruit crop of the arid and semiarid region. In the present investigation two types of DNA based marker RAPD and ISSR were used to characterize 8 date palm genotypes grown in the Kutch region. Amplification of genomic DNA of 8 genotypes using 13 RAPD analyses yielded 88 fragments, of which 35 were polymorphic, with an average of 2.69 pol...

Pleurotus eryngii, known as king oyster mushroom has been widely used for nutritional and medicinal purposes. This study was initiated to screen the suitable conditions for mycelial growth and to determine the phylogenetic relationship of the selected strains. Optimal mycelial growth was observed at 30℃ and minimum mycelial growth observed at 10℃. This mushroom tolerates a broad pH range for my...

ziziphora tenuior, named kakooti in farsi, is one of the aromatic and medicinal plants of a wide distribution in iran. in this study, rapd molecular markers, (by utilization of 16 primers) was applied to determine the genetic diversity of 39 samples of ziziphora tenuior from 21 geographical regions in iran. a number of 100 polymorphic bands out of 110 amplified ones was produced and analyzed th...

DNA-based techniques have great potential for detecting genetic variability between genotypes. However, each approach generates different molecular markers, so the kind and amount of polymorphism detected and the cost and time required vary among them. In general, a good method for DNA fingerprinting to detect genetic polymorphism must generate a complex pattern of bands per experiment, the res...

Genetic diversity among ginger genotypes collected from different parts of the country was studied using molecular markers (30 RAPD and 55 SSR). Compared to primers SSR were efficient in distinguishing genotypes. A total 86 23 polymorphic bands observed with primers, respectively. Percentage polymorphism between 97.40 % 56.54 %. Grouping by combined data indicated that irrespective their place ...

A total of 31 strains of Vibrio cholerae O1 (10 from outbreak cases and 7 from surface water) and non-O1 (4 from clinical and 10 from surface water sources) isolated between 1993 and 1997 were examined with respect to presence of cholera enterotoxin (CT) gene by PCR-based assays, resistance to antibiotics, plasmid profiles and random amplified polymorphic DNA (RAPD) analysis. All were resistant...

Genetic diversity of two wild Kalibaus, Labeo calbasu populations and one hatchery stock was studied using random amplified polymorphic DNA (RAPD) method. The three 10–mer random primers (OPA01, OPB02 and OPC03) yielded a total of 26 reproducible and consistently scorable RAPD bands of which 15 (57.69%) were considered as polymorphic (P95) indicating a high level of genetic variation in all the...

it is important to identify the genetic diversity and phylogenic relationships for achieving desirable citrus cultivars. in present investigation rapd markers were used to determine genetic diversity among 29 citrus genotypes. from 19 used primers, 248 polymorphic bands were amplified. primers opa-07 and opa-05 (with 19 and 8 amplified bands), produced maximum and minimum polymorphic bands, res...