Genetic diversity and relationships among 6 Amaranthus species from 8 phytogeographic regions of the Indo-Gangetic plains were analyzed using a random amplified polymorphic DNA (RAPD) marker. RAPD primers yielded a total of 262 amplicons, ranging from approximately 250 to approximately 3000 bp in size with an average of 13.1 amplicons per primer, of which 254 amplicons (96.94%) were polymorphic...

Various organisms have been characterized by molecular methods, including fungi of the genus Cryptococcus. The purposes of this study were: to determine the discriminatory potential of the RAPD (Random Amplified Polymorphic DNA) primers, the pattern of similarity of the Cryptococcus species, and discuss their useful application in epidemiological studies. We analyzed 10 isolates of each specie/...

The present study was conducted to evaluate the genetic diversity and relationships of 5 Egyptian indigenous strains (Fayoumi, Dokki-4, Golden Montazah, Silver Montazah, and El-Salam) using 10 random amplification of polymorphic DNA (RAPD) primers. The gel analyses were performed using GelCompare II where the matrix was constructed followed by the cluster analysis for the matrices. Three hundre...

RAPD markers were used to reveal the genetic diversity of the genus Camptotheca. Three primers (OPA02, OPA03, and OPA04), generating 44 polymorphic bands using randomly amplified polymorphic DNA (RAPD) markers, were able to discriminate among 25 Camptotheca populations. The band size varied from 268-4,411bp, with an average of 15 bands/primer. Of these populations, cultivars ‘Katie’ (KT) and ‘A...

Precise investigation of genetic diversity by means of novel molecular tools has made it possible to identify the superior genotypes among various male and female pistachio populations. Cytogenetic studies have shed light on the possible presence of distinct sex chromosomes in male and female genotypes. In this study, 22 start codon targeted (SCoT) primers were used to investigate the genetic d...

The genetic variation and population structure of three populations of Anopheles darlingi from Colombia were studied using random amplified polymorphic markers (RAPDs) and amplified fragment length polymorphism markers (AFLPs). Six RAPD primers produced 46 polymorphic fragments, while two AFLP primer combinations produced 197 polymorphic fragments from 71 DNA samples. Both of the evaluated gene...

Aspergillus parasiticus and Aspergillus sojae are two morphologically similar species belonging to the Aspergillus section flavi. A new method to distinguish the two species, the aflatoxin producer A. parasiticus and the koji mold A. sojae, was developed. Single primers with arbitrary sequences were used to generate random amplified polymorphic DNA (RAPD) markers from strains of these two speci...

In the present study, fresh Silkmoths were used for DNA extraction. The wings, legs and antennae of moths were cut with sterile scissors, crushed under liquid nitrogen to fine powdered content which yielded high resolution DNA bands using modified phenol chloroform extraction method. The genomic DNA from twelve different silkmoth genotypes was further checked for its suitability in DNA fingerpr...

The aim of this study was to apply RAPD technique to analyze the genetic variability among the Iranian CA-MRSA isolates.The RAPD amplification was implemented on 25 strains isolated from the anterior nares of 410 healthy children using four randomly selected oligonucleotide primers from the stocks available in our laboratory, including the primers 1254, GE6, OLP6 and OLP13 from our stock. The a...