The colicin A lysis protein, Cal, is synthesized at the same time as colicin A by Escherichiu coli harbouring plasmid pColA after induction by mitomycin C. Its function in the induced bacteria involves the release of colicin A, quasi-lysis, the death of the producing cells and the activation of the outer membrane phospholipase A. We have found that these various functions are affected different...

Cold plasma and ozone sanitation of irrigation solutions can oxidize both microbes non-target micronutrients because their high oxidation-reduction potential (ORP) is a non-selective mode action. The objective this study was to evaluate the effects cold treatment on oxidation iron manganese in nutrient containing one four chelates (iron-ethylenediaminetetraacetic acid (Fe-EDTA), iron-diethylene...

Objective: This study aims to estimate and compare the time-course change in blood glucose levels by blood cells in serum, and plasma with or without preservatives, which may reflect the rate of glucose utilization by blood cells. Method: This laboratory-based cross-sectional study was carried out using a blood specimen of 28 participants among which 14 were diagnosed with diabetes and 14 were...

Phosphotriesterase (PTE) activities in mammalian serum are typically found in the lipoprotein fraction. This PTE requires Ca++ for activity and is consequently inactivated by ethylenediaminetetraacetic acid (EDTA). There is also a little known PTE in mammal serum that is resistant to EDTA inactivation. In this work, the PTE activities for the substrates O-hexyl O-2, 5-dichlorophenyl phosphorami...

OBJECTIVE This study aims to compare the clot stabilization on root surfaces conditioned with citric acid and ethylenediamine-tetraacetic acid (EDTA). MATERIALS AND METHODS Scaled root samples (n = 100) were set in fve groups: group I-control group (saline solution); group II (24% EDTA); group III (25% citric acid); group IV (EDTA + citric acid); group V (citric acid + EDTA). Fifty samples we...

Purified human serum butyrylcholinesterase after treatment with either of the metal chelators EDTA or NaCN was able to bind to a Zn(2+)-chelate-Sepharose affinity column and was eluted from the column by EDTA or imidazole. Prior EDTA treatment of the enzyme was essential for binding to this affinity column. The enzyme could be labelled with (65)Zn(2+) after EDTA treatment of the enzyme. Diethyl...

BACKGROUND Chelation therapy with sodium edetate (EDTA) improved renal function and slowed the progression of renal insufficiency in patients subjected to lead intoxication. This study was performed to identify the underlying mechanism of the ability of EDTA treatment to protect kidneys from damage. METHODS The effects of EDTA administration were studied in a rat model of acute renal failure ...